Instruction Manual

48 VR NxT| Instruction Manual 1.0 02-2019|VRNXTinstr1EN|© 2019 Menarini Silicon Biosystems SpA
ANNEX B: DEPArray RECOVERIES
PREPARATION FOR DOWNSTREAM GENETIC
ANALYSIS
Prepare the DEPArray recoveries for downstream genetic
analysis with VR NxT
Sample characteristics
Note: Samples prepared according to this protocol can be used for downstream analysis methods such as Ampli1 WGA Kit or
similar.
Cells are collected from the DEPArray following the rules below:
Number of cells Number of drops*
one one
up to 20 two
more than 20 six
Note*: Number of cell collection drops from DEPArray.
Precautions
The liquid reduction of samples coming from the DEPArray should be performed within 24 hours from the end of the
recovery process.
The DEPArray rack is tilted sideways and it is not suitable for the centrifuge. Therefore samples must be moved to the
centrifuge racks while preparing the sample for volume reduction.
Procedure
1. Take the recovery rack with the tubes from the DEPArray.
2. Place it on a flat surface and close the tubes.
3. Move the tubes from the DEPArray rack to the centrifuge racks.
4. Place the same number of tubes on each rack to guarantee balance.
5. Insert the centrifuge racks in the swing bucket centrifuge.
6. Centrifuge the samples as follows:
If samples come from... And contain up to... Then centrifuge at...
DEPArray V2
1 drop
l (live cells) 300 g x 3 min
l (fixed cells) 1,000 g x 3 min
2 drops l (live cells) 300 g x 10 min
l (fixed cells) 1,000 g x 10 min
6 drops
DEPArray NxT 1 drop l (live cells) 300 g x 3 min
l (fixed cells) 1,000 g x 3 min
2 drops
6 drops l (live cells) 300 g x 10 min
l (fixed cells) 1,000 g x 10 min
7. Place the centrifuge racks under the laminar flow hood.