Instruction Manual

ANNEX A: CELLSEARCH sample preparation for DEPArray
VR NxT| Instruction Manual 1.0 02-2019|VRNXTinstr1EN|© 2019 Menarini Silicon Biosystems SpA 47
7. Take 1,000 μl of DABUF from the DABUF bottle.
8. Put the pipette tip against the wall in the upper part of the Sample tube.
9. Carefully push the knob to release the DABUF and let the liquid flow down the wall of the tube.
10. Discard the tip.
11. Centrifuge the Sample tube at 1,000 g for 5 minutes at room temperature in a swing bucket centrifuge.
12. Carefully remove the Sample tube from the centrifuge and move it under the biosafety cabinet class II.
Wash the sample for the second time
1. Attach a clean P1000 tip to the P1000 pipette.
2. Carefully remove the supernatant from the Sample tube until leaving ~150 μl of liquid.
3. Transfer the removed supernatant in the S2 tube.
4. Discard the tip.
Prepare the sample for the volume reduction
1. Take a P200 pipette and attach a clean P300 tip to it.
2. Push the pipette knob to the first stop.
3. Aspirate the entire volume from the Sample tube slowly releasing the pipette knob.
4. Lean the pipette tip against the bottom of a MicroAmp Reaction Tube with cap.
5. Without creating bubbles, push the pipette knob to release the sample in the same MicroAmp Reaction Tube with
cap while moving the pipette upward slowly.
NOTICE: In case of bubbles, the output sample volume is not guaranteed.
6. Discard the tip.
7. Put the tube in the centrifuge rack and centrifuge it at 1,000 g x 5 min with a swing bucket centrifuge:the sample is
now ready for the volume reduction (see "Volume reduction to 13.5 or 12.5 µl" on page28).