Instruction Manual
Table Of Contents
- Glossary of terms
- Assistance
- Information on this manual
- Safety
- Reception
- Product description
- Getting started
- Operating with VR NxT
- Maintenance of the instrument
- Troubleshooting and errors
- Technical features
- ANNEX A: CELLSEARCH sample preparation for DEPArray
- ANNEX B: DEPArray recoveries preparation for downstream genetic analysis
- License Agreement
- Conformity
- Warranty and Responsibility
40 VR NxT| Instruction Manual 1.0 02-2019|VRNXTinstr1EN|© 2019 Menarini Silicon Biosystems SpA
TECHNICAL FEATURES
Technical data
Features
Throughput (cycle time) l DEPArray IN and High Volume: 4 samples in ~25 s
l DEPArray OUT and Low Volume: 4 samples in ~7 s
Permitted consumable DA0644 (provided in a box of 72 pcs, REF: DA0665)
Permitted holder l Blue holder (DA0649)
l Gray holder (DA0648)
Max rotational speed 3600 rpm
Maximum kinetic energy 10 J
Time to stop the instrument l Quick stop: < 250 ms
l Controlled stop:< 3 s
Sample container 200 µl PCR tube (see "Required materials and tools (not included)" on page43
and "Compatible tubes" on page26)
Sample input volume From 1 to 4 tubes, max 200 µl in each tube
Sample input number of cells l DEPArray IN: ≤ 40,000 cells
l DEPArray OUT: ≤ 507 cells
l Low Volume: ≤ 10,000 cells
l High Volume: ≤ 100,000 cells
Sample output volume l DEPArray IN program:~12.5 µl
l DEPArray OUT program:~2 µl
l High Volume: ~13.5 or ~12.5 µl depending on buffer type and sample
container
l Low Volume: ~3 or ~2 µl depending on buffer type and sample container
Sample output volume repeatability Depends on buffer type, sample container and selected program. Typical
standard deviation ~0.35 µl.
Cell retention yield l DEPArray OUT:typically ≥ 90%
l DEPArray IN:typically ≥ 85%
Permitted buffers for processing l DABUF
l RPMI
l DMEM
l F12K
l Running Buffer (for Low Volume and High Volume only)
Max permitted density of the liquid and
material for processing
1.2 g/mL
Permitted biological sample Fixed or live cells of biological risk group II or lower
Instrument lifetime 7 years
Physical features
Dimensions
Power supply excluded:
l Width: 30 cm (11.8")
l Depth: 15 cm (5.9")
l Height:15 cm (5.9")
Weight 5 kg (11 lb)
Clearance 30 cm (11,8") around the instrument
A-Weighted emission sound pressure
level at 30 cm from the instrument
70.2 dB (A)
Communication system l Frequency: 13.56 MHz
l Max power of the antenna for the RFIDtag: +4.4 dBuA/m