Instruction Manual

Operating with VR NxT
VR NxT| Instruction Manual 1.0 02-2019|VRNXTinstr1EN|© 2019 Menarini Silicon Biosystems SpA 33
Reduce the volume to 13.5 or 12.5 µl if the volume is similar to the starting
level
1. Take each tube with the tweezers (see "Remove the tubes from the holder" on page22), move it to a common tube
storage rack and close it (see "Close the removed tubes" on page22).
2. Move the tubes in the centrifuge racks.
3. Centrifuge the tubes using a swing bucket centrifuge.
4. "Prepare the closed holder" on page28.
5. "Load VR NxT" on page29.
6. "Reduce the volume to 13.5 or 12.5 µl" on page29.
Reduce the volume to 3 or 2 µl if the volume is similar to the starting level
1. Take each tube with the tweezers (see "Remove the tubes from the holder" on page22), move it to a common tube
storage rack and close it (see "Close the removed tubes" on page22).
2. Move the tubes in the centrifuge racks.
3. Centrifuge the tubes using a swing bucket centrifuge.
4. "Prepare the closed holder" on page30.
5. "Load VR NxT" on page30.
6. "Reduce the volume to 3 or 2 µl" on page30.
Reduce the volume to 13.5 or 12.5 µl if the volume is similar to the intended
final level
1. Take each tube with the tweezers (see "Remove the tubes from the holder" on page22), move it to a common tube
storage rack and close it (see "Close the removed tubes" on page22).
2. Move the tubes in the centrifuge racks.
3. Centrifuge the tubes using a swing bucket centrifuge.
4. Move the tubes under the biosafety cabinet class II.
5. Add 100 µl of manipulation buffer (see "Permitted buffers for processing" on page40).
6. Centrifuge the tube again using a swing bucket centrifuge.
7. "Prepare the closed holder" on page28.
8. "Load VR NxT" on page29.
9. "Reduce the volume to 13.5 or 12.5 µl" on page29.
Reduce the volume to 3 or 2 µl if the volume is similar to the intended final
level
1. Take each tube with the tweezers (see "Remove the tubes from the holder" on page22), move it to a common tube
storage rack and close it (see "Close the removed tubes" on page22).
2. Move the tubes in the centrifuge racks.
3. Centrifuge the tubes using a swing bucket centrifuge.
4. Move the tubes under the biosafety cabinet class II.
5. Insert 100 µl of 1X PBS in each tube with a pipette and close the tubes.
6. Insert the centrifuge racks in the swing bucket upstream centrifuge.
7. Centrifuge the sample using a swing bucket centrifuge: the sample is now ready for the volume reduction to ~2 µl.
8. "Prepare the closed holder" on page30.
9. "Load VR NxT" on page30.
10. "Reduce the volume to 3 or 2 µl" on page30.