Instruction Manual

Table Of Contents

Operating with VR NxT

Volume reduction to 3 or 2 µl

Warnings

l Do not lean on the instrument.

l Do not stay longer than necessary within the 30 cm of clearance from the instrument.

l Do not deposit any potentially hazardous material within the 30 cm of clearance from the

instrument.

Notices about the input sample

NOTICE: Avoid inserting samples with bubbles in VR NxT. In case of bubbles, the output sample volume is not

guaranteed (see "Troubleshooting" on page37 to solve the problem).

NOTICE: The samples to be inserted in VR NxT must have been processed using a swing bucket upstream centrifuge

with plates.

NOTICE: If the sample to be processed comes from DEPArray, see "Prepare the DEPArray recoveries for downstream

genetic analysis with VR NxT" on page48 to prepare it for VR NxT.

Prepare the closed holder

CAUTION! To guarantee correct sample processing, only use VR NxT holder and cap and inspect them

before the use (see "Inspect the holder/closed holder" on page18).

1. Once centrifuged, place the samples under the biosafety cabinet class II.

2. Place the Gray holder (DA0648) in the holder rack (see "Position the closed holder" on page21).

3. Place the holder rack with the holder under the biosafety cabinet class II.

4. Open the tubes and insert them in the holder one at a time. Start from number 1 position indicated on the holder.

5. Insert each tube cap in its tube cap cavity to align them (see "Insert the tubes" on page19).

6. Put a new cap on the holder and push the holder clips to close the holder.

NOTICE: Do not remove, damage or write on the RFID tag of the cap. A cap without the RFID tag or with an

unreadable RFID tag is unserviceable.

7. Ensure the closed holder to be inserted perfectly in the holder rack.

Load VR NxT

1. Place the rack with the closed holder on a flat surface next to the instrument.

2. If the instrument is off, press the main power switch to turn it on:the instrument performs a self-diagnostic test.

3. Open the rotor chamber.

4. Ensure there is no foreign body in the rotor chamber (see "Inspect the instrument" on page17).

5. Insert the closed holder in its housing and push it down until it clicks (see"Insert the closed holder in the instrument"

on page20).

6. Close the rotor chamber.

Reduce the volume to 3 or 2 µl

NOTICE: Before starting the program, please make sure to read the available stop procedures (see "Stop the volume

reduction process" on the facing page).

1. Act as follows:

If you are reducing the sample

volume...

Then... And display...

for general purposes press or Low Volume

for using DEPArray DEPArray OUT

2. Press to confirm the program: the instrument performs an internal check.

3. If Ready appears and the status LED flashes, press to start the program:the status LED turns steady. If Not

ready appears and the status LED is off, press for more information and solve the problem.