Instruction Manual

Table Of Contents

Operating with VR NxT

3. Place the holder rack with the holder under the biosafety cabinet class II.

4. Open the tubes and insert them in the holder one at a time. Start from number 1 position indicated on the holder.

5. Insert each tube cap in its tube cap cavity to align them (see "Insert the tubes" on page19).

6. Put a new cap on the holder and push the holder clips to close the holder.

NOTICE: Do not remove, damage or write on the RFID tag of the cap. A cap without the RFID tag or with an

unreadable RFID tag is unserviceable.

7. Ensure the closed holder to be inserted perfectly in the holder rack.

Load VR NxT

1. Place the rack with the closed holder on a flat surface next to the instrument.

2. If the instrument is off, press the main power switch to turn it on:the instrument performs a self-diagnostic test.

3. Open the rotor chamber.

4. Ensure there is no foreign body in the rotor chamber (see "Inspect the instrument" on page17).

5. Insert the closed holder in its housing and push it down until it clicks (see"Insert the closed holder in the instrument"

on page20).

6. Close the rotor chamber.

Reduce the volume to 13.5 or 12.5 µl

NOTICE: Before starting the program, please make sure to read the available stop procedures (see "Stop the volume

reduction process" on page31).

1. Act as follows:

If you are reducing the sample

volume...

Then... And display...

for general purposes press or High Volume

for using DEPArray DEPArray IN

2. Press to confirm the program: the instrument performs an internal check.

3. If Ready appears and the status LED flashes, press to start the program:the status LED turns steady. If Not

ready appears and the status LED is off, press for more information and solve the problem.

4. At the end of the program, open the rotor chamber.

NOTICE: Do not open the rotor chamber while the program is running and the status LED is steady.

5. Move the closed holder in the holder rack.

6. Close the rotor chamber.

7. Move the holder rack with the closed holder under the biosafety cabinet class II.

8. Push the bottom of the holder clips to open the closed holder.

9. Lift the cap and throw it in the biohazard waste disposal container.

NOTICE: To avoid the liquid leakage, handle the cap with care.

Note: A small liquid drop may be present out of the tube (e.g. see the image below). In this case, the output volume is

guaranteed anyway.

10. Take each tube with the tweezers (see "Remove the tubes from the holder" on page22), move it to a common tube

storage rack and close it (see "Close the removed tubes" on page22).

Note: For the DEPArray NxT, resuspend the sample and load it in the DEPArray NxT cartridge. For DEPArray V2, add 1 µl

of manipulation buffer, resuspend the sample and load it in the DEPArray V2 cartridge.