Instruction Manual

28 VR NxT| Instruction Manual 1.0 02-2019|VRNXTinstr1EN|© 2019 Menarini Silicon Biosystems SpA
Operating with VR NxT
Programs for using DEPArray:sample characteristics
NOTICE: The correct trap of the liquid in the cap is guaranteed only if the input sample volume is respected.
NOTICE: The output sample volume is guaranteed only if the sample does not contain bubbles.
The following table shows the characteristics of the samples for each program intended for using DEPArray.
Program
name
MicroAmp Reaction Tube with cap Holder
required
Input
volume
Number of
cells
Buffer composition Output
volume
DEPArray
IN
max 200
µl
≤ 40,000 l DABUF or
l RPMI with 10% FBS or
l DMEM with 10% FBS or
l F12K with 10% FBS
~12.5 µl
DEPArray
OUT
max 200
µl
≤ 507 fixed
cells
DABUF and 1X PBS (PBS concentration range 72-
88%)
~2 µl
≤ 85 live
cells
1X PBS (PBS concentration range 85-88%) and
one of the following buffer:
l RPMI with 10% FBS or
l DMEM with 10% FBS or
l F12K with 10% FBS
Volume reduction to 13.5 or 12.5 µl
Warnings
l Do not lean on the instrument.
l Do not stay longer than necessary within the 30 cm of clearance from the instrument.
l Do not deposit any potentially hazardous material within the 30 cm of clearance from the
instrument.
Notices about the input sample
NOTICE: Avoid inserting samples with bubbles in VR NxT. In case of bubbles, the output sample volume is not
guaranteed (see "Troubleshooting" on page37 to solve the problem).
NOTICE: The samples to be inserted in VR NxT must have been processed using a swing bucket upstream centrifuge
with plates.
NOTICE: If the sample to be processed comes from CELLSEARCH, see "Prepare the CELLSEARCH sample for
DEPArray with VR NxT" on page45 to prepare it for VR NxT.
Prepare the closed holder
CAUTION! To guarantee correct sample processing, only use VR NxT holder and cap and inspect them
before the use (see "Inspect the holder/closed holder" on page18).
1. Once centrifuged, place the samples under the biosafety cabinet class II.
2. Place the Blue holder (DA0649) in the holder rack (see "Position the closed holder" on page21).