Instruction Manual
Table Of Contents
- Glossary of terms
- Assistance
- Information on this manual
- Safety
- Reception
- Product description
- Getting started
- Operating with VR NxT
- Maintenance of the instrument
- Troubleshooting and errors
- Technical features
- ANNEX A: CELLSEARCH sample preparation for DEPArray
- ANNEX B: DEPArray recoveries preparation for downstream genetic analysis
- License Agreement
- Conformity
- Warranty and Responsibility
Operating with VR NxT
VR NxT| Instruction Manual 1.0 02-2019|VRNXTinstr1EN|© 2019 Menarini Silicon Biosystems SpA 27
Please comply with the following table to obtain the volumes indicated in this Instruction Manual.
Program
MicroAmp Reaction Tube
with cap
MAXYMum Recovery
Thin Wall PCR Tube with
flat cap
for general
purposes
for using DEPArray
Programs for general purposes:sample characteristics
NOTICE: The correct trap of the liquid in the cap is guaranteed only if the input sample volume is respected.
NOTICE: The output sample volume is guaranteed only if the sample does not contain bubbles.
The following table shows the characteristics of the samples for each program intended for general purposes.
Program
name
MicroAmp
Reaction Tube
with cap
MAXYMum Recovery
Thin Wall
PCR Tube with flat cap
Holder
required
Input
volume
Number
of
cells
Buffer
composition
Output
volume
Input
volume
Number
of
cells
Buffer
composition
Output
volume
High
Volume
max
200 µl
≤
100,000
l DABUF
or
l RPMI or
l DMEM
or
l F12K or
l Running
Buffer
~12.5 µl max
200 µl
≤
100,000
l DABUF
or
l Running
Buffer or
l RPMI or
l DMEM
or
l F12K
~13.5
µl
Low
Volume
≤ 1,000 l DABUF
or
l Running
Buffer or
l RPMI or
l DMEM
or
l F12K
~2 µl ≤
10,000
l DABUF
or
l Running
Buffer or
l RPMI or
l DMEM
or
l F12K
~3 µl