User`s guide
Chapter 8 Maintenance and Troubleshooting
8-20 PerSeptive Biosystems
8
Calibration (mass) shifted 
up or down by 10 Da 
Uneven matrix layer 
causing hot and cold 
spots
Acquire a number of 
spectra and accumulate 
scans.
Prepare new sample spot.
Resolution labels or 
signal-to-noise not 
displayed
Peaks not detected Apply peak detection 
when acquisition is 
complete by clicking   
in the toolbar.
Table 8-2 Instrument Control Panel Troubleshooting (Continued)
Symptom Possible Cause Action
Table 8-3 PSD Troubleshooting
Symptom Possible Cause Action
Cannot see low mass ions 
in PSD mode
Guide Wire Voltage% too 
high
Adjust. See 
Section 5.4.3.3, 
Optimizing Guide Wire 
Voltage%.
Peak selected with 
Precursor Ion Selector not 
appearing, or not 
appearing at expected 
mass (single-stage mirror 
only)
Timed ion selector not 
working, due to invalid 
flight length value
Call PerSeptive 
Biosystems Technical 
Support.










