User`s guide

Chapter 6 Acquiring Mass Spectra
6-72 PerSeptive Biosystems
6
Internal/External
Calibration
Calibration type to use for this row. Click the down arrow and
select one of the following:
• Blank—No calibration applied during processing.
Acquisition calibration (calibration specified in .BIC) is
persisted.
• External—Applies the constants in the .CAL file specified to
the data file acquired in this row.
• Internal—Applies the constants in the .CAL file if specified,
generates new calibration constants using the reference
masses specified in the .SET file, and applies the new
constants to the data file acquired in this row. For more
information, see Section 6.7.7.4, Internal Standard
Calibration Considerations.
• Internal-Update—Applies the constants in the .CAL file, if
the specified file exists, generates new calibration constants
using the reference masses specified in the .SET file,
applies the new constants to the data file acquired in this
row, and saves the new constants in the .CAL specified. For
more information, see Section 6.7.7.3, Performing Close
External Calibration.
For information, see:
• Section 6.7.1, Understanding Settings,
Macros, and Calibration
• “Creating calibration (.CAL) files” on page 6-66
NOTE: Calibration specified in the Sequence Control Panel is
performed after calibration performed by the .BIC file used to
acquire the data file. Calibration performed by the Sequence
Control Panel overrides the calibration performed by the .BIC
file.
NOTE: If the .BIC file for the row specifies a save/accumulation
mode that creates more than one data file, calibration is
performed on all data files created.
Table 6-4 Run List Parameters (Continued)
Field Description