User`s guide

Chapter 5 Optimizing Instrument Settings
5-46 PerSeptive Biosystems
5
5.3.4 Understanding Guide Wire Voltage%
By applying voltage to the beam guide wire (Figure 5-10), you
can overcome the dispersion effect from the source and
refocus ions on the detector.
Figure 5-10 Beam Guide Wire
All models In general:
• Increase Guide Wire Voltage to increase sensitivity.
• Decrease Guide Wire Voltage to increase resolution. A
higher Beam Guide Wire voltage can negatively impact
resolution, because it is focusing ions that would not
otherwise be reaching the detector.
For more information, see “Guide Wire Voltage%” on
page 5-18.
Linear mode • For Linear mode analysis of peptides in the 1,000 to
2,000 Da range, a setting of 0.05 to 0.1 percent is
adequate.
• For Linear mode analysis of ions over 20,000 Da range,
start with a setting of 0.3 percent and decrease as
needed.
• Settings below 0.02 percent may not give adequate
sensitivity.
Guide Wire Voltage applied,
ions focused on detector
Detector