User`s guide

Chapter 5 Optimizing Instrument Settings
5-40 PerSeptive Biosystems
5
Grid Voltage% Linear/
Reflector
Critical parameter with optimum value for
maximum resolution.
Digitizer Bin size
(nanoseconds)
Linear/
Reflector
Decreasing improves resolution.
NOTE: Decreasing Bin size increases file
size.
Digitizer Input
Bandwidth (not
available with Signatec
digitizers)
Linear/
Reflector
Decreasing may improve signal-to-noise
ratio.
Increasing may improve the resolution.
Accelerating Voltage Linear Increasing improves sensitivity and
resolution, but is limited by other factors
such as the digitization rate.
Reflector Decreasing to 10,000 to 15,000 V can
improve resolution for compounds
<2,000 Da.
Laser intensity Linear/
Reflector
Increasing improves signal-to-noise ratio.
Increasing too high increases
fragmentation, and resolution is sacrificed
due to saturation.
Decreasing too low decreases sensitivity.
Shots/
Spectrum
Linear/
Reflector
Increasing improves signal-to-noise ratio.
Parameter Mode Impact