User`s guide

Sample Plate Types Supported
Voyager
™
Biospectrometry
™
Workstation User’s Guide 3-57
3
Guidelines for
creating .PLT files
Note the following:
• The diameter of a position on a plate is determined by the
plate used:
• If you are using welled or laser-etched plates, the
position diameter is determined by the well or
laser-etched position size (2,540 µm on a 100-well
plate).
• If you are using plates with polished blank
surfaces, the position diameter is determined by
the size of the physical sample spot delivered by
manual application or automated sample
preparation device.
• When creating a .PLT file, specify absolute X,Y
coordinates that accommodate the sample diameter, but
do not allow samples to overlap.
• After you create a .PLT file, create an .SP file to
accommodate the sample diameters defined in the .PLT
file. The DEFAULT.SP file is set to analyze 2,540-µm
diameter positions, corresponding to standard sample
positions on a 100-well plate.
NOTE: For optimum mass accuracy, do not spot sample on
outer rows of the plate. See Figure 3-11 on page 3-56.
Guidelines for
creating .SP files
When creating .SP files to analyze sample positions defined in
custom .PLT files, note that the area available for analysis
around the absolute X,Y coordinate of the position specified in
the .PLT file is determined by three factors:
• Plate being used
• Tolerances and non-systematic errors
• Search pattern radius
Search pattern files are described in detail in Section 6.6.4,
Search Pattern Files.