User`s guide

Loading Samples on Sample Plates
Voyager
™
Biospectrometry
™
Workstation User’s Guide 3-31
3
3.2.2 Loading Samples
(Dried Droplet Application)
NOTE: If you are loading a plate for acquisition or
automated sample positioning in Automatic Control mode,
use a laser-etched or welled sample plate to provide a
reference point for sample application.
When to use Use this technique for most applications, when sample
concentration is >0.1 pmol/ µl.
Location of
standards
For optimum mass accuracy with welled plates or plates with
laser-etched indentations, place standards in wells or
indentations adjacent to the samples for which you are
calibrating. Do not use the outer wells on the sample plate.
when using flat or polished blank plates.
Loading samples 1. Create a log sheet listing sample position and sample
name. Appendix D, Log Sheets, contains a master
sheet that you can copy and use.
NOTE: For optimum mass accuracy, do not use the
outer rows of the sample plate, unless using internal
standards.
2. Using a clean pipette tip for each new sample, load the
following:
• If you premixed samples—Load 0.5 to 2 µl of
sample/matrix solution on the appropriate position.
• If you are mixing sample and matrix on the
sample plate—See “Mixing sample and matrix on
the sample plate” on page 3-27.
• If you prepared samples with ZipTips—Load 0.5
to 2 µl of sample/matrix solution on the appropriate
position.