User`s guide

Chapter 3 Preparing Samples
3-16 PerSeptive Biosystems
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You can load polymer samples on sample plates in two ways:
• Thin layer polymer method (yields even response,
but provides adequate sample response for only
10 laser shots)—Load 0.3 µl sample/matrix solution in
one sample position.
• Thick layer polymer method (yields less even
response, but provides adequate sample response
for 50 to 100 laser shots)—Load 2 to 3 µl sample/matrix
solution in one sample position by placing the pipet tip in
the sample position and slowly expelling while keeping
the tip in contact with the sample plate.
NOTE: In general you should not touch the pipette tip
to the sample plate surface. During the thick layer
method it is necessary to touch the tip to the sample
plate when using THF as the solvent, in order to slowly
expel the sample onto the sample plate.