User`s guide

Table of Contents
x PerSeptive Biosystems
Chapter 7 PSD Analysis
7.1 Overview of PSD Analysis......................................................... 7-2
7.1.1 Post-Source Decay Analysis .................................................. 7-2
7.1.2 Segments and Composite Spectra ......................................... 7-6
7.1.3 PSD Data Files ...................................................................... 7-7
7.1.4 Mass Calculation for Fragment Ions ....................................... 7-8
7.1.5 Optimizing the Precursor Ion Selector .................................... 7-9
7.2 Enhancing Fragmentation with CID
............................................ 7-11
7.3 Acquiring PSD Data with Standard Instrument Settings (.BIC) Files
.... 7-17
7.3.1 Determining the Precursor Ion Mass .....................................7-19
7.3.2 Determining the Number of Segments to
Acquire for a Complete Composite Spectrum ........................7-21
7.3.3 Setting PSD Acquisition Parameters .....................................7-24
7.3.4 Filling in the Segment List .....................................................7-28
7.3.5 Acquiring and Saving PSD Segments ...................................7-32
7.4 Exploring PSD Mode
.............................................................. 7-36
7.4.1 Observing the Effects of Laser Intensity ................................7-37
7.4.2 Observing the Effects of Precursor Ion Selector ....................7-40
7.4.3 Observing the Effects of Grid Voltage% ................................7-44
7.4.4 Summary ..............................................................................7-47
7.5 Viewing PSD Data
.................................................................. 7-48
Chapter 8 Maintenance and Troubleshooting
8.1 Maintenance.......................................................................... 8-2
8.1.1 Maintenance Schedule ........................................................... 8-2
8.1.2 Hardware Maintenance .......................................................... 8-3
8.1.3 Backing Up and Archiving Data .............................................. 8-6
8.2 Troubleshooting
...................................................................... 8-7
8.2.1 Spectrum Troubleshooting ..................................................... 8-7
8.2.2 Software Troubleshooting .....................................................8-19
8.2.3 Hardware Troubleshooting ....................................................8-22