User Guide

1. Click on a gene from the results list of
either pairwise or project analysis.
2. At the bottom of the One-Click Gene
Summary window, click on Perform
Sequence Analysis.
3. A new window will open with the
selected sequence pasted in a form
ready for BLAST analysis. Select the
type of BLAST search to perform
(blastn or blastx) from the buttons at
the bottom left corner of the window.
4. To determine suitable primers for a
selected nucleotide sequence, there is
a link to primer3, again with the
sequence already loaded into the
application.
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Note: Use blastn to BLAST the sequence
directly against a nucleotide database. Use
blastx to BLAST against a protein
database. The latter works by translating
your nucleotide sequence in all six reading
frames (three for each direction) and
blasting each result against the protein
database.
BLAST
®
Analysis
A BLAST search can be performed on any
gene sequence within the secure GeneSifter
environment.