User Guide

Uploading Data
Using FlexLoad Wizard (continued)
10. Provide the column number or letter
that contains the gene ID.
11. Identify the type of gene ID by
selecting either Auto Detect,
Accession Number, IMAGE Clone
ID, or Other. If Accession Number
or IMAGE Clone ID is selected and
the data file contains other identifiers
or blank rows, errors may occur. In
general Auto Detect should be
used.
12. Optionally, indicate the column
number for gene annotation, if
available.
13. Indicate the row number where the
intensity data begins. Do not include
the column headings.
14. Provide the column numbers that
contain the respective data. For
example, if your data file contains
the cy3 intensity in column 8 and
column 10 contains the background,
enter 8 for Col 1 and 10 for Col 2 to
specify these values for the cy-3
intensities.
Op refers to the operation to perform
between the two values. Op may be
used to subtract background, or take
the ratio of foreground/background
for quality control purposes. It is not
necessary to enter any value for Op,
or Col 2.
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