UltiMate® 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides January 2010 Rev.
Table of Contents 1. Introduction ...................................................................................................................... 1 1.1 How to Use this Manual ............................................................................................. 1 1.2 Definition of LCi ........................................................................................................ 2 1.3 Typical System Setups ..............................................................................
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 6.3.2 Dual-stack with Extension Kit ............................................................................. 32 6.4 Flow Schematics ...................................................................................................... 33 6.4.1 Single-stack without Extension Kit ...................................................................... 33 6.4.2 Dual-stack with Extension Kit ..........
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 1. Introduction 1.1 How to Use this Manual This document is provided as a quick installation guide for the Dionex UltiMate® 3000 LCi solutions.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 1.2 Definition of LCi Dionex has developed a number of “Intelligent LC” (LCi) solutions to allow analysts to obtain the best possible combination of performance, reliability and ease-of-operation of their HPLC system(s).
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 1.3.2 Dual-Stack Whenever bench space is of no concern but reduced system height is more important to users, the system can be configured as a dual-stack. Several different combinations are conceivable, depending on the kind and number of modules included in a system.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Figure 4: Schematic for tightening a Viper fitting Attach Viper to the target thread and tighten the screw slowly until you feel the very first resistance. This is the 0° mark. Do not use tools other than the black knurl for opening and closing a connection. Tighten the screw clockwise to an angle between 0° and 45° and start operating your LC system at the desired working pressure.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 1.5 Viper Capillary Kits and Packaging 1.5.1 Capillary Kits for Single Stacks All UltiMate 3000 RS systems are delivered with a dedicated set of 3 Viper capillaries for system plumbing. In addition, dedicated Viper capillary RS and SD kits containing 3 capillaries each are available to equip Dionex UltiMate 3000 systems with Viper.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 1.5.1.1 Scope of Delivery 1.5.1.1.1 Viper Capillary Kit, RS System Table 1: Scope of delivery for the Viper capillary kit, RS system (P/N 6040.2301) Description Quantity Viper capillary (SST), ID x L 0.18 mm/0.007” x 450 mm 1 pc. Viper capillary (SST), ID x L 0.13 mm/0.005” x 350 mm 1 pc. Viper capillary (SST), ID x L 0.13 mm/0.005” x 250 mm 1 pc.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 1.5.2 LCi Capillary Kits All Viper LCi capillary kits are delivered in a high-quality box with a foam inlay. Independent of the ordered kit, you will always receive the same box, accommodating all necessary accessories and capillaries for your individual LCi kit. Figure 7: Packaging box for all Viper LCi capillary kits. January 2010 Rev.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 1.6 Viper Labeling Each Viper capillary comes with a labeling clip. This clip can be easily detached and reattached to 1/16” and 1/32” OD capillaries. In addition, a set of 5 blank clips and stickers is supplied for individual labeling purposes. Figure 8: Removable labeling clip with sticker for individual capillary labeling. Page 8 Rev.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 1.7 General System Preparation Allow the system modules to adapt to ambient temperature for 4 hours to allow for potential condensation to evaporate. Do not connect the units to the mains during this period. If condensation is still detected after 4 hours, allow the system to continue to warm up to ambient temperature without connecting it to the mains, until all condensation is completely gone.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Filter holder with filter frit Retaining guide Figure 10: Handling of the eluent filter frit Note: To exchange the tubing, first remove the frit, and then remove the eluent tube before removing the retaining guide. Important: Always use eluent tubing with filter frits for drawing eluents from the eluent bottles. This prevents contamination of the HPLC system.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 1.9 Using 10-Port Instead of 6-Port TCC-3x00 Valves The installation steps for the Automated Application Switching, On-line Sample Preparation and Parallel LC capillary kits in this document refer to the use of 2-position 6-port switching valves. However, TCC-3x00 column compartments equipped with 2-position 10-port switching valves can also be used for these applications.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Page 12 Rev.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 2. Viper™ Tandem Operation Capillary Kits, DualTernary Standard (P/N 6040.2804) or Rapid Separation (P/N 6040.2803) 2.1 Benefits of Tandem Operation Tandem operation is an LCi solution for increasing the throughput of gradient separations between 50% and 100%.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 2.3 Stacking the Modules Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 2.4 Flow Schematic The figure below shows the schematic setup of the tandem operation configuration. Install your Viper capillaries according to the information of Table 4 and the system stack schematic ( 2.3).
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Note: Page 16 In case of a dual-stack setup, the TCC 10-port valve right turns into a TCC 10-port valve left. Both stacks should have a distance of about 7 cm (2.8 inches). Connecting a post-column cooler with a stackable Dionex detector does not work without additional capillaries when setting up the system in the U-configuration (1.3.2). Rev.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 3. Viper™ Application Switching Capillary Kits, DualTernary Standard (P/N 6040.2806) or Rapid Separation (P/N 6040.2805) 3.1 Benefits of Application Switching Automated Application Switching is an LCi solution for increasing system productivity by running two applications consecutively and independently on one UltiMate 3000 system.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 3.3 Stacking the Modules Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 3.4 Flow Schematic The figure below shows the schematic setup of the application switching configuration. Install your Viper capillaries according to the information of Table 6 and the system stack schematic ( 3.3). Figure 13: Flow schematic of the automated application switching setup January 2010 Rev.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Table 6: Detailed description of capillary connections of the automated application switching setup Description of Connections Capillary Dimensions (ID x L) PumpLeft outlet – TCC 10-port valve right, port 2 0.18 mm/0.007” x 850 mm PumpRight outlet – TCC 10-port valve right, port 4 0.18 mm/0.007” x 750 mm TCC 10-port valve right, port 1 – Column 1 inlet RS: 0.13 mm/0.005” x 350 mm SD: 0.18 mm/0.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 4. Viper™ On-Line SPE Capillary Kits, Dual-Ternary Standard (P/N 6040.2802) or Rapid Separation (P/N 6040.2801) 4.1 Benefits of On-line SPE Automated on-line solid phase extraction is an LCi solution allowing for easy and automated isolation of analytes from a complex matrix. The automated on-line SPE reduces time, labor and cost, and thus increases productivity.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 4.3 Stacking the Modules Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 4.4 Flow Schematic The figure below shows the schematic setup of the on-line SPE configuration. Install your Viper capillaries according to the information of Table 8 and the system stack schematic ( 4.3).
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Note: Page 24 In case of a dual-stack setup, the TCC 6-port valve right turns into a TCC 6-port valve left. Both stacks should have a distance of about 7 cm (2.8 inches). Connecting a post-column cooler with a stackable Dionex detector does not work without additional capillaries when setting up the system in the U-configuration (1.3.2). Rev.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 5. Viper™ Parallel LC Capillary Kits, Dual-Ternary Standard (P/N 6040.2810) or Rapid Separation (P/N 6040.2809) 5.1 Benefits of Parallel LC Parallel LC is an LCi solution for increasing the throughput for both isocratic and gradient separations by efficient use of hardware. The Parallel LC system uses two pumps and two detectors, and shares one autosampler and one column compartment.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 5.3 Stacking the Modules Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 5.4 Flow Schematic The figure below shows the Parallel LC setup. With this configuration, two Chromeleon instruments (“time bases”) share the column compartment and the autosampler. The autosampler is alternated between two parallel flow paths to allow for synchronized analyses on two individual columns. Two detectors independently and simultaneously acquire the related signals.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Table 10: Detailed description of capillary connections of the Parallel LC setup (dual-stack) Description of Connections Capillary Dimensions (ID x L) PumpLeft outlet – TCC 6-port valve left, port 6 0.18 mm/0.007” x 550 mm PumpRight outlet – TCC 6-port valve left, port 4 0.18 mm/0.007” x 350 mm TCC 6-port valve left, port 5 – WPS sampler valve, port 5 RS: 0.13 mm/0.005” x 250 mm SD: 0.18 mm/0.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 6. Viper™ Automated Method Scouting Kits, Quaternary Standard (P/N 6040.2808) or Rapid Separation (P/N 6040.2807) 6.1 Benefits of Automated Method Scouting Automated Method Scouting is a powerful solution for the convenient determination of optimized analytical method parameters, such as pH, temperature, stationary phase, and solvent composition.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Description Quantity Capillary clip for color-coding, white 30 pcs. Capillary clip for color-coding, red 30 pcs. Capillary clip for color-coding, black 30 pcs. Capillary clip for color-coding, blue 30 pcs. Capillary clip for color-coding, green 30 pcs. Laminated flow scheme for Automated Method Scouting 1 pc.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 6.3 Stacking the Modules 6.3.1 Single-stack without Extension Kit Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 6.3.2 Dual-stack with Extension Kit Please stack the modules as shown below. The picture is a schematic to illustrate the course of the flexible Viper capillaries in general. Dionex recommends installing all capillaries inside the modules as needed. For the use of the Method Scouting Extension Kit, a dual-stack is recommended.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 6.4 Flow Schematics 6.4.1 Single-stack without Extension Kit The figure below shows the schematic setup of the Method Scouting configuration as a single-stack, supporting up to four different eluents. Install your Viper capillaries according to the information of Table 14 and the system stack schematic ( 6.3.1).
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Table 14: Detailed description of capillary connections of the Method Scouting setup (single-stack) Description of Connections Capillary Dimensions (ID x L) Pump outlet – WPS sampler valve, port 5 0.18 mm/0.007” x 450 mm WPS sampler valve, port 4 – TCC 7-port valve right, port 7 (center) RS: 0.13 mm/0.005” x 350 mm SD: 0.18 mm/0.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides 6.4.2 Dual-stack with Extension Kit The figure below shows the schematic setup of the Method Scouting configuration with the extension kit as a dual-stack, supporting up to 13 different eluents. Install your Viper capillaries according to the information of Table 15 and the system stack schematic ( 6.3.2).
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Table 15: Detailed description of capillary connections of the Method Scouting setup with extension kit (dual-stack) Description of Connections Capillary Dimensions (ID x L) Pump outlet – WPS sampler valve, port 5 0.18 mm/0.007” x 450 mm WPS sampler valve, port 4 – TCC 7-port valve right, port 7 (center) RS: 0.13 mm/0.005” x 650 mm SD: 0.18 mm/0.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Parts Table 16: List of orderable part numbers Description P/N Viper SST Flex.-Cap., ID × L 0.13 mm/0.005” x 65 mm, SST 6040.2307 Viper SST Flex.-Cap., ID × L 0.13 mm/0.005” x 150mm, SST 6040.2315 Viper SST Flex.-Cap., ID × L 0.13 mm/0.005” x 250mm, SST 6040.2325 Viper SST Flex.-Cap., ID × L 0.13 mm/0.005” x 350mm, SST 6040.2335 Viper SST Flex.-Cap., ID × L 0.13 mm/0.005” x 450mm, SST 6040.
® UltiMate 3000 Viper™ Capillary Kits for Intelligent LC (LCi) System Solutions Quick Installation Guides Description P/N Eluent bottle, 1 L 2270.0012 Retaining guide for solvent tubing 6000.0042 Sealing plug for eluent bottle screw cap 6000.0047 Capillary labeling clips for 1/16” and 1/32” OD capillary, 10 pcs. 6040.2399 Filter frit, SST, porosity 10 μm 6268.0110 Viper union for direct connection of two Viper capillaries 6040.2304 Viper plug, SST 6040.
