Valco Instruments Co. Inc. M Series Pump Models M6 and M50 Instruction Manual m6-m50.indd Rev. 2/15 North America, South America, and Australia/Oceania contact: ® Valco Instruments Co. Inc. 800 · 367· 8424 713 · 688· 9345 713 · 688· 8106 valco@vici.com sales tech fax Europe, Asia, and Africa contact: ® VICI AG International Schenkon, Switzerland Int + 41 · 41 · 925· 6200 Int + 41 · 41 · 925· 6201 info@vici.
The information in this manual has been carefully checked and believed to be accurate; however, no responsibility is assumed for inaccuracies. VICI maintains the right to make changes without further notice to products described in this manual to improve reliability, function, or design. VICI does not assume any liability arising from the use or application of this product; neither does it convey any license under its patent rights. and are trademarks of Intelligent Motion Systems, Inc.
Table of Contents Product Description Operating Principle..........................................................................................1 Software...........................................................................................................1 Getting Started Components of the M Series System..............................................................2 PC System Requirements................................................................................
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1 Product Description The Cheminert® M Series liquid handling pump is a syringe-free pump capable of delivering a bidirectional flow to six orders of magnitude. It is a positive displacement pump, which means that it is self-priming and tolerant of any gas which may find its way into the fluid lines. Since there is no separate fill cycle, the pump can be operated continuously, and volumetric capacity is limited only by time.
2 Getting Started Components of the M Series System Check the contents of the packages against Figure 1 to verify that you have received all of the components. Contact the factory if anything is missing or damaged. (NOTE: damaged shipments must remain with the original packaging for freight company inspection.
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4 Hardware Installation Mounting The mounting points of the pump motor and controller are given in Figure 2, along with the external dimensions of each module. For best results, the M Series pump must be oriented with its ports facing up. If the pump is oriented in any other direction, bubbles may be trapped in the internal chambers of the pump. The controller should be mounted in such a way to allow adequate cooling. If the cooling fan is obstructed the controller may overheat.
5 Connections CAUTION: Supply power to the power supply only after all connections have been made. Failure to do so may destroy the controller. Take precautions to avoid static discharges to the controller. 1. Connect the power supply and motor power cable to the controller as shown below. 2. Connect the motor power cable to the pump. 3. Plug one end of the RS-232 cable into the pump controller and the other into a serial port on the computer. 4.
6 Initial Setup M6-LHS Software Installation Insert the M6-LHS software CD into the PC. If the installation doesn’t begin automatically, browse the CD and double click on the file called setup.exe. The installer will place an icon for M6-LHS on the Windows desktop. COM Port Assignment 1. Double click the icon on the desktop to start the M6-LHS software. 2. On main menu, choose Utility > Setup, as in Figure 4. 3. Select the correct COM port for the pump and valve. If no valve is being used, select None.
7 Selecting Pump and Gearbox Values In the Gearbox pulldown menu, select CP-DSM1 for Model M6 or CP-DSM2 for Model M50. In the Pump pulldown menu, select 100 for Model M6 or 625 for Model M50. (The values 4 and 750 are currently unused.) These values represent nominal µL/revolution for the two models. The next section discusses how these can be overridden for greater accuracy.
Initial Setup 8 To override the factory default values for backlash and µL/rev: 1. Locate the backlash and µL/rev values on the QC certificate. 2. Click the Enable box under Backlash on the Setup screen, and type in the backlash value. 3. Click the Use measured uL/rev box under Pump, and type in the volume per revolution. 4. Click Apply, then Close.
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10 Priming and Testing the Pump At this stage, some basic tests will determine that the tubing connections are tight and that the pump is rotating in the proper direction. The tests include an initial priming of the pump to remove all air and unwanted solutions in the tubing. Use proper safety precautions when pumping flammable liquids. In all applications, make sure you have enough tubing and sufficient quantities of solutions ready for use before beginning a procedure.
11 click THE box for a one time fixed volume aspirate or dispense. leave the box unclicked for continuous pumping at the entered flow rate. Figure 9: Priming the pump in the Test Mode (M6 values shown) 1. If the screen in Figure 9 is not already open, go to the main menu, and choose Utility > Test. 2. If the tubing ID is 0.010” or less, enter Flow Rate: 200 µL/min Fixed Volume: 1000 µL Click the Enable box If the tubing ID is larger than 0.
12 Programming Application Methods Method Screen Functions From the main menu, choose Method > New to open the screen below. 1: Method status Idle, Active (running), or Editing 2: Mode activation buttons Run: Starts running the method. Reset: Pauses a method which is underway, allowing a change to the editing mode. Repeat: If this box is clicked, when the method completes the last action, it goes back to first action in a continuous loop.
13 6: Action line A step in the method sequence, reflecting the values entered by selecting or typing values in the Pump, Valve, and Wait fields. To move to a different line for editing, just click on it or use No. 12: Up and Dn buttons. 7: Pump function Aspirate: Draw liquid into the line connected to the pump outlet (Port B). Dispense: Force a pre-programmed amount of liquid through the pump.
Programming Application Methods 14 Example Application Methods In the Test Mode, user-entered flow parameters cannot be saved; when you exit the software, the values revert to defaults. So creating several simple example methods is a useful way to start learning the M6-LHS software. The methods below utilize flow parameters characteristic of the Model M6, but the same principles apply to programming for the M50. Example method 1: “Prime System” 1.
Programming Application Methods 15 8. Use the Dn (down) button to highlight the last line, then click the Insert button. 9. For the newly inserted line, select Dispense in the pump function field, then enter 110 for the volume and 1000 for the flow rate. 10. Click the End Edit button. 11. On the main menu, choose Method > Save, and call this method “aspirate and dispense”. The result should look like Figure 11.
Programming Application Methods 16 Example method 3: “Dispense Selected Reagents” This method, which employs an 8-position multiposition valve for selecting multiple reagents to be added to tubes or microplates, is a typical example of the applications possible when a Valco or Cheminert valve is integrated into the pump / M6-LHS system. A wash step (or in this case, a prime step) is incorporated between reagents.
17 Maintenance Follow these recommended cleaning and maintenance instructions to obtain optimum performance and maximum life from your pump. If the pump is not going to be used for at least 24 hours, make sure no solvents are left in the pump. Following this procedure will extend pump lifetime and prevent seizing. Routine maintenance • Flush the pump thoroughly with distilled or de-ionized water after each use. Store the pump dry. • Avoid running the pump dry for extended periods.
18 Chemical Compatibility of Wetted Surfaces To ensure best results, please consider the chemical compatibility of the wetted surfaces with the liquids to be used before putting the pump into service. Model M6 PTFE The piston tips are made from PTFE. Polytetrafluoroethylene is the generic name for the class of materials such as Teflon®. It offers superior chemical resistance and lends itself to good sealing characteristics.
19 Model M50 Valcon H2 The M50 cap is a carbon fiber-reinforced, PTFE-lubricated inert polymer. This material is routinely used at 5000 psi at temperatures not more than 75°C. Ceramic The M50 pump rotor is made of ceramic (ZrO2), which is resistant to most chemicals. PTFE The piston tips are made from PTFE. Polytetrafluoroethylene is the generic name for the class of materials such as Teflon®. It offers superior chemical resistance and lends itself to good sealing characteristics.
20 Technical Support Technical support for this product is handled by the VICI facilities in Houston, Texas, USA, and Schenkon, Switzerland. USA Telephone: Fax: Email: (713) 688-9345 (713) 688-8106 tech_usa@vici.com Switzerland Telephone: Fax: Email: int+41 41 925-6200 int+41 41 925-6201 tech@vici.ch Firmware updates for the MicroLynx-4 can be downloaded from www.IMSHome.com. Returning Pumps for Repair The following procedure must be followed when pumps are returned to VICI for repair: 1.
21 Operational and Technical Specifications ¤ ¢ ¢ ££
22 Appendix A: MicroLYNX Terminal Section Terminal Programming Mode The Terminal (MicroLYNX) programming mode is for advanced control of the M Series Pump, allowing the user to alter the factory default settings for various parameters. While this may be useful in certain research applications, the main use is for OEM (original equipment manufacturer) customers. Figures 13 and 14 show the various control settings used for specialized applications, as well as the default settings.
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24 Appendix B: Setup for Multipump Operation Connections Make up cables using the following guidelines. Note that communication with the PC is assumed to be via RS-232. If you are using an RS-232 to RS-485 converter, treat the converter as the Host, with all MicroLYNXes configured as slaves.
25 Setting the Address of each MicroLYNX The party address can be set by either hardware switches or software. In this discussion, “party address” and “drive name” are synonymous. Hardware method 1. Connect the communications cable (RS-232 or RS-485) to the first MicroLYNX only. (NOTE: power can be connected to all units.) 2. Set the three address switches to OFF (which equals “no address”). 3. Apply power. You should get the following startup string.
Setup for Multipump Operation 26 Software method The hardware method is the simplest approach, and should be used when possible. 1. Connect the communications cable (RS-232 or RS-485) to the first MicroLYNX only. (NOTE: power can be connected to all units.) 2. Set the three address switches to OFF (which equals “no address”). 3. Apply power. You should get the startup string shown on page 23, step 3. 4. The address is set with the DN command.
Setup for Multipump Operation 27 12. Set the address with the DN command. Input: nDN=“x”[Ctrl-J] The n represents the address assigned by the hardware switches in step 10. The “x” represents the address you are assigning via the software method, which can be any single ASCII character from a-z, A-Z, or 0-9. The address must be enclosed with quotes during the naming process. 13. Save the address. Input: xSAVE[Ctrl-J] 14. Set all three address switches to OFF.
Setup for Multipump Operation 28 Troubleshooting Party Mode Communications If you can’t communicate with a MicroLYNX in party mode, first make sure that commands are preceded with the correct address and terminated with [Ctrl-J]. Also remember that the unit will stop listening if you send a wrong address. Send a [Ctrl-J] and try again. If it still doesn’t work, read on. If using hardware switch addressing • Are you sending a lower case address? The address is uppercase A-G.
Setup for Multipump Operation 29 If problems still exist, perform the following: 1. Check all cable connections to ensure there are no loose wires or short circuits or incorrect connections. 2. Remove power. 3. Connect the communication cable to the problem MicroLYNX only. 4. Set all address switches on the problem MicroLYNX to OFF. 5. Apply power. 6. Set the MicroLYNX to single mode. Input: Output: *PARTY=0 (The MicroLYNX will echo the address to which it is currently set.
30 Warranty Certain applications using the M Series pump may involve potential risks of death, personal injury, or severe property or environmental damage. The M Series pump is not designed, authorized, or warranted to be suitable for use in life support devices or systems or other critical applications. Inclusion of VICI products in such applications is understood to be fully at the customer’s risk.
