Ultraviolet Crosslinkers Operating Instructions and Service Manual IMPORTANT: Please read these instructions before operating your UVP UV Crosslinker to familiarize yourself with its operation.
UV CROSSLINKERS UVP CONTENTS 1. Introduction 1 2. Important Safety Information 1 3. Description and Specifications 1 4. Operation 3 5. Applications 6 6. Maintenance, Care and Cleaning 7 7. Changing the UV Wavelength 8 8. Calibration Procedure 10 9. Replacement Parts 12 10.
UV CROSSLINKERS 1. UVP INTRODUCTION UVP Crosslinkers offer researchers an instrument to quickly, safely and efficiently expose samples to a controlled amount of ultraviolet radiation. CL-1000 series features a lay-down type drawer while the CX-2000 has a pull-out drawer. Exposure of samples to UV provides for the following: • • • • • • • Crosslinking of DNA or RNA to nitrocellulose, nylon or reinforced nitrocellulose. PCR sample contamination control.
UV CROSSLINKERS UVP Features • • • • • • • • • • • • Microprocessor controlled/UV sensor feedback system Multiple set functionspreset UV energy exposure preset UV time exposure user set UV energy exposure user set UV time exposure Maximum UV energy exposure setting of 999,900 microjoules per cm2 Maximum UV time exposure setting of 999.
UV CROSSLINKERS 4. UVP OPERATION 1. Place the crosslinker on a level work surface. Be sure there is enough room in front for the door to open easily. 2. Plug the female end of the power cord into the crosslinker. 3. Plug the male end of the power cord into a properly grounded electrical outlet. The proper operating voltage of the crosslinker is found on the product information label.
UV CROSSLINKERS UVP The new setting will appear on the LED display. Push ENTER on the touchpad. The new setting is now installed. B. Preset ultraviolet time exposure Push PRESET and then TIME on the tactile touchpad. The red light at each position should now be lit and the preprogrammed UV exposure time of 2 minutes should be displayed in the LED. Push START on the touchpad. After a slight delay to energize the UV tubes, the LED will begin to countdown.
UV CROSSLINKERS UVP Push START on the touchpad. After a slight delay to energize the UV tubes, The LED will begin to countdown. the unit will stop automatically at the end of the exposure cycle and will beep five times. Exposure is now complete. D. User set UV time exposure Setting your own UV time exposure can be achieved as follows: Push the TIME on the tactile touchpad and then set your requirements by pushing the numbers. Your time exposure settings will be displayed on the LED in flashing mode.
UV CROSSLINKERS 5. UVP APPLICATIONS The Crosslinker is a multi-purpose ultraviolet exposure instrument for use in the laboratory. A wide variety of applications for ultraviolet radiation exist in the laboratory. A. UV crosslinking of DNA and RNA by covalently binding nucleic acids to transfer membranes nitrocellulose, nylon or nylon-reinforced nitrocellulose membranes after Northern, Southern, slot or dot blotting.
UV CROSSLINKERS 6. UVP MAINTENANCE, CARE AND CLEANING OF THE CROSSLINKER The UVP UV Crosslinker is built to provide you with trouble-free operation. To ensure correct service: NOTE: ALWAYS UNPLUG UNIT FROM ELECTRICAL SUPPLY BEFORE CLEANING OR DRYING. 1. Wipe ANY water from inside and outside the unit with a soft cloth or sponge. 2. Use soap and water with a soft cloth or sponge to clean the unit. 3. Do not allow chemicals to remain on unit surfaces. 4.
UV CROSSLINKERS 7. UVP CHANGING THE UV WAVELENGTH A UV Crosslinker is purchased with shortwave, longwave or midrange tubes. However, if user requirements and applications change, the Crosslinkers provide you with the unique ability to change the ultraviolet wavelength and recalibrate the UV sensor and microprocessor to the new UV wavelength. This is accomplished by purchasing FIVE tubes of the NEW UV wavelength and the proper UVP calibration sensor.
UV CROSSLINKERS UVP 11. The previous calibrated operation of the crosslinker will continue following any unsuccessful recalibration. Replace the new UV wavelength tubes with the previously removed old wavelength tubes. 12. Successful recalibration to the NEW UV wavelength changes the values in the microprocessor and numeric setting value is displayed on the LED. To return to original or another wavelength the proper UV calibration sensor is needed.
UV CROSSLINKERS 8. UVP CROSSLINKER CALIBRATION PROCEDURE Equipment necessary: Chart recorder Two probes with banana plug adapters One radiometer with UVX-25 calibrated sensor Cable with banana plug and jack adaptor to connect radiometer to chart-recorder Power source (100V, 115V or 230V) Procedure: 1. Connect the crosslinker to the power source. Do not turn on the unit at this time. 2.
UV CROSSLINKERS 9. UVP Run the Crosslinker in calibration mode by depressing the STOP/RESET button and turning the power switch on at the same time. At this point, both pens should start recording information on paper. Sometimes either pen will record zero information regardless of the lights in the crosslinker being on, if this is the case, check for bad connections. 10. During calibration, check the voltage levels on both pens.
UV CROSSLINKERS 9. UVP REPLACEMENT PARTS AND SERVICING PROCEDURES PART NO. A. OPERATIONS WITHOUT REMOVING THE TOP COVER Changing tubes B.
UV CROSSLINKERS UVP 10. GENERAL SERVICING PROCEDURES A. Operations Without Removing The Top Cover Changing the Tubes It is recommended to change all FIVE 8-Watt 254nm UV tubes at the same time. The unit does not require recalibration if you are replacing the old sources with new sources of the same wavelength. 1. Purchase five tubes from UVP. 2. Unplug the unit from the electrical supply. 3.
UV CROSSLINKERS UVP 5. Cut all of the cable ties coming from the tube holder following the two wires from the tube holder to where it terminates. Note: there will be two different termination points for each tube holder. Once this has been located, cut the wires (marking them to ensure easy replacement). 6. The tube holder(s) can now be removed by pushing the holder into the main chamber and feeding the wires through the cut-out. 7. Replace tube holder(s). (Part number 36-0003-01) 8.
UV CROSSLINKERS UVP 6. To secure the starter holder, place the holder in your right hand and with your left hand in the chamber, press the now loosened reflector adjacent to the holder inwards. This will push the black spigots inwards thereby securing the starter holder. 7. Re-fit the reflector. Replacement of fuse holders These are located at the back of the unit just above the mains connector. There are two fuses in separate holders. Replacement is the same for both. 1.
UV CROSSLINKERS UVP Replacement of UV sensor UVP recommends returning the Crosslinker to the UVP factory for service. This is located on the right hand side of the internal chassis assembly at the back near the base. 1. Unplug the sensor connector from the PCB board marked J2. 2. Release the sensor by removing the location screw with a screwdriver and holding the nut inside the main chamber with a spanner. Pull sensor clear. 3. For replacement, reverse the above procedure.
UV CROSSLINKERS UVP C. Operations With The Internal Chassis Assembly Removed Removal of the assembly 1. Release the wire to the micro switch located on the bottom left internal chassis assembly. 2. Release the main power cable to ballasts - black wire with connector (color red). 3. Release all eight nuts positioned four on top of the unit, two sets on either side of the unit. See diagram for positions. 4. Slide unit out from the main frame.
UV CROSSLINKERS E. UVP General Items Changing fuses The fuses are located on the back panel and are marked with a self-adhesive sticker. 1. Turn fuse cap 15 degrees anti-clockwise. 2. Pull fuse and cap from the holder. 3. Pull fuse from the cap. 4. Replace the fuse. 5. Replace fuse cap and fuse into holder and turn 15 degrees clockwise. Changing plastic feet The feet are fitted to the bottom of the cabinet. They are removed by unscrewing the screw mounted inside each foot.
UV CROSSLINKERS UVP Schematic Wiring Diagram - 19 -
UV CROSSLINKERS UVP Internet: www.uvp.com Corporate Headquarters: UVP, Inc. 2066 W. 11th Street Upland, CA 91786 Tel: (800)452-6788 or (909)946-3197 Fax: (909)946-3597 / E-Mail: info@uvp.com European Operations: Ultra-Violet Products Ltd. Unit 1, Trinity Hall Estate, Nuffield Road Cambridge CB4 1TG United Kingdom Tel: +44(0)1223 420022 Fax: +44(0)1223 420561 / E-Mail: uvp@uvp.co.
