User guide

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Glossary
Glossary
Artifact: In imaging, a flaw caused either by the imaging process or by the hardware itself. For
example, dust on the camera lens could cause small bright or dark spots in an image.
Aspect Ratio: The ratio between an image's width and its height. If the aspect ratio is not
preserved, the image will appear stretched or squashed.
Bits: The smallest units of computer measurement. A bit is a single binary value (i.e. it can be
"on" or "off" only). Bits typically are combined into units of eight, called "bytes." Modern computer
processors work with groups of 4 ("32-bit processor") or 8 ("64-bit processor") bytes at a time.
BMP: Microsoft Bitmap image file format. BMP is a lossless format which provides some
compression to reduce file size. BMP files generally have a BMP extension.
Control Handle: A small square at the corner (or similar point) of a graphical object that marks its
extent and indicates that the object is selected. Usually the object can be resized by dragging the
control handle; in some cases, different behavior results.
Electrophoresis: The movement of suspended particles through a fluid or gel through the
application of electrical current to the suspension medium.
Fidelity: The degree to which an image is true (i.e. accurate and uncorrupted) to the original
scene it represents. Also used in audio technology with the same meaning.
GIF: Graphic Interchange Format, a proprietary Xerox image compression format. GIF is a lossy
compression format that results in very small files. Files stored in GIF usually have a GIF
extension.
Image Depth: The size (and thus range) of intensity numbers supported per pixel in an image.
Intensity: The measure of brightness of a pixel. In a monochrome image, each pixel has a single
intensity. In a colored image, each pixel has three intensities: one for red; one for green; and one
for blue. The actual intensity values depend on an image's depth.
JPEG: A common lossy compression image format used to store images on disk. JPEG files
generally have JPG or JPEG extensions.
Lossless Compression: Compression schemes that preserve the image's integrity in full.
Generally, lossless compression results in much larger files than lossy compression on the same
image.
Lossy Compression: Compression schemes that tolerate some pixel value changes to make the
image compress to a smaller size. Because the changes are irreversible, the image has "lost"
some of its original detail after such an operation.
Macro Mode: Close-up mode for a digital camera or web-camera. Macro mode is usually
appropriate for imaging microbiology slides.
Microbiology: The branch of biology dealing with microscopic forms of life.
Microscopy: The use of or investigation with a microscope.
Monochrome: Black-and-white, with shades of gray.
Pixel: Short for "picture element." A pixel is a single dot in a computer image. The dot has a
certain color (for a color image) or an intensity (for a monochrome image). For a more detailed
explanation, see Inside a Pixel.