iBox® Anesthesia System Instruction Manual UVP, LLC 2066 W 11th Street, Upland, CA 91786 Tel: (800) 452-6788 / (909) 946-3197 Fax: (909) 946-3597 Ultra-Violet Products Ltd. Unit 1, Trinity Hall Farm Estate, Nuffield Road Cambridge CB4 1TG UK Tel: +44(0)1223-420022 / Fax: +44(0)1223-420561 Web site: www.uvp.
iBox Anesthesia System 2 IMPORTANT! Read this manual before setting up and operating the iBox Anesthesia System! The user should be thoroughly familiar with the contents of this manual prior to using the system with animals. Only technicians who are properly certified to use isoflurane/sevoflurane vaporizer and anesthetic equipment should operate the iBox Anesthesia System.
iBox Anesthesia System 3 Introduction to the iBox Anesthesia System The iBox Anesthesia System is designed for the anesthesia of small animals. The system is a small portable unit that has been designed for maximum efficiency, ease of use and low anesthetic gas consumption. The system delivers a precisely blended mixture of oxygen and isoflurane or sevoflurane into a plastic induction chamber, offering ultra-efficient anesthetizing of animals.
iBox Anesthesia System 4 Components of the iBox Anesthesia System 5 6 8 1 2 7 1) 4 3 Five-Port Breathing Device/Manifold: The Five-Port Breathing Device/Manifold is designed to maintain anesthesia in the iBox Scientia and iBox Spectra for up to five mice or one rat at a time. The inflow tube delivers anesthetic gas from the vaporizer unit to the nosecones, while the outflow tube directs expended gas from the nosecones to the charcoal filter canister.
iBox Anesthesia System 5) 5 Isoflurane/Sevoflurane Vaporizer: The vaporizer is a precision instrument that blends oxygen and either isoflurane or sevoflurane to the proper ratio, and then outputs it to the system components. The gas blend is adjusted with a large locking dial on top of the unit. The vaporizer outputs agent concentrations from 0-5% in .5% graduations. NOTE: UVP does not ship isoflurane or sevoflurane with this system. Contact a lab supply company to purchase isoflurane or sevoflurane.
iBox Anesthesia System 6 Setup of iBox Anesthesia System The following is a schematic diagram of the five-port iBox Scientia and iBox Spectra anesthesia system. The specific components (letters) of this schematic will be referenced parenthetically throughout this manual. Note: The only difference between this schematic and the anesthesia system for the iBox Explorer is the replacement of a single-port breathing device/manifold in place of the five-port component shown below.
iBox Anesthesia System 2. 7 Attach the Oxygen Source to the Vaporizer (Figure 2): a. To connect the oxygen regulator (B) to the vaporizer (C), use the green oxygen hose. b. Attach the green oxygen hose from the regulator output to the vaporizer stand input at the back of the vaporizer stand. Figure 2: Attach Oxygen Source to Vaporizer Stand Input 3. 4. 5. 6. Connect the Induction Chamber: a.
iBox Anesthesia System 8 from the warming plate (see red circles, Figure 5). Then, attach the single nosecone to the breathing device/manifold and insert the black plug into the nosecone to prevent gas from escaping into the darkroom when not in use. Inside the iBox Darkroom 1. Connect the hose on the breathing device/manifold (J) marked ‘To Inlet’ (Figure 6) to the fitting marked ‘Inlet’ mounted inside the iBox. 2.
iBox Anesthesia System 9 Connecting the Warming Plate 1. For the iBox Scientia and Spectra, place the warming plate (Figure 8) on the lift platform inside the darkroom. Plug one end of the power cord into the back of the warming plate and the other end to an outlet at the (inside) top of the darkroom. See the iBox Scientia and iBox Spectra User Manuals for more information. 2. The warming plate is preset to a temperature of 37°C +2. 3.
iBox Anesthesia System 10 Operating the iBox Anesthesia System Prior to operation of the system, be sure that all components are properly connected as directed in Setup of iBox Anesthesia System. Weigh charcoal filter canisters with each use. Replace the charcoal filter after 100 grams of increased weight. The vaporizer should be in the off position and the oxygen regulator dial turned completely off. WARNING: A high isoflurane-to-oxygen ratio can result in harm or death to animals.
iBox Anesthesia System 9. 11 Ensure that the warming plate is plugged into the Accessory outlet in the back of the iBox Scientia and Spectra darkroom. The warming plate in the iBox Explorer is always on as long as the system is on. 10. To turn the iBox warming plate on, flip the I/O (on/off) switch to the I (on) position. The warming plate will show the temperature of the warming plate in the display. 11.
iBox Anesthesia System 12 Helpful Tips Oxygen flow rates and isoflurane/sevoflurane concentrations in this manual are meant only as guidelines. Operators should determine their own values based on observation and proper training. During longer procedures, it is important to continually monitor the animals in the iBox for proper depth of anesthesia. It may be necessary to reduce the isoflurane/sevoflurane concentration by 0.5 (%) during hours-long procedures.
iBox Anesthesia System 13 Animals are too deeply anesthetized: Usually in this case the isoflurane/sevoflurane concentration on the vaporizer is too high. If an animal is observed to be in danger of dying, reduce the isoflurane/sevoflurane concentration to zero and let pure oxygen flow until the animal returns to a normal state of anesthesia. Then, turn the isoflurane concentration to a lower value than previously used. Breathing should be even and not appear labored.
iBox Anesthesia System 14 If the labels on the breathing device are missing, the breather inlet is the hose connected to the white silicone duckbill valves inside the breather port. The breather exhaust is the hose connected to the black holes inside the breather port. If all of the instructions have been followed yet problems continue to arise, the vaporizer or oxygen regulator may be out of calibration.
iBox Anesthesia System 15 Care and Cleaning Vaporizer Every two weeks, the vaporizer should be drained into appropriately-marked containers and disposed of properly. Less frequent intervals may be used when the anesthetic does not contain additives or a stabilizing agent. WARNING: Do not drain the vaporizer into a container that is not properly marked. Draining the Vaporizer: 1. The control dial should be in the OFF position. 2.
iBox Anesthesia System 16 Service Procedures Replacement Parts and Accessories Replacement parts and accessories are available for use with the iBox Anesthesia System. Find UVP’s contact information listed under “Technical Support” below. Description Part Numbers ReFresh Charcoal Filter Canister (Qty.
iBox Anesthesia System 17 Warranty Euthanex equipment and products are warranted against defects in material and workmanship under normal use and operation for a period of one year from date of shipment. Contact Euthanex for further details.
