DigiDoc-It® Imaging System Installation and User Instructions UVP, LLC Ultra-Violet Products Ltd. 2066 W. 11th Street Upland, CA 91786 Phone: (800) 452-6788 / (909) 946-3197 Fax: (909) 946-3597 Unit 1, Trinity Hall Farm Estate Nuffield Road, Cambridge CB4 1TG UK Phone: +44(0)1223-420022 Fax: +44(0)1223-420561 Web Site: www.uvp.
DigiDoc-It Imaging System 2 Introduction The DigiDoc-It Imaging System is a basic gel imaging system designed to fit over any of UVP’s optional High Performance UV Transilluminators or optional Benchtop UV Transilluminator and drawer combination. The system combines: Hood enclosure DigiCam 125 digital color camera Ethidium Bromide (EtBr) Filter ® Doc-It LS Acquisition Software (refer to software manual for installation) UV transilluminator or drawer assembly (optional).
DigiDoc-It Imaging System 2 Camera & UV Filter The digital color camera includes a zoom lens. The EtBr filter, orange-colored UV blocking band pass filter, is installed inside the hood at the UVP factory. The filter is used to absorb UV and IR radiation from the transilluminator and to enhance the orange/pink bands generated by Ethidium Bromide stained gels. The filter can be removed when imaging non-fluorescent media (protein gels, colony plates, etc.) in order to produce brighter images.
DigiDoc-It Imaging System 3 System Setup DO NOT ATTEMPT TO CONNECT ANY WIRING WHILE THE EQUIPMENT IS CONNECTED TO ANY POWER SUPPLY. CAUTION: Do not install the system in places with high moisture, dust or high temperature. Do not use any oil or petroleum based cleaner for the cabinet. Use only mild soap or detergent solution for cleaning. Ensure that the system is turned OFF during cleaning. Keep the equipment away from motors or other large magnetic equipment apparatus.
DigiDoc-It Imaging System 3. 4. 4 To connect the USB cable to the camera, thread the camera’s USB cable through the round notched hole from the back of the camera cap and plug into the camera. Leave the other end unplugged until ready to load the software. Plug the camera’s power supply into a wall outlet or surge-protector receptacle. Note: The Ethidium Bromide filter is installed inside the hood enclosure at the UVP factory. If the filter needs to be replaced, turn the hood upside down.
DigiDoc-It Imaging System Connect the Camera to the Computer NOTE: It is recommended to only connect the camera to the computer while the camera is off. 1. 2. Connect the USB cable from the camera to the computer. Turn the camera on and select A-DEP on the camera dial. NOTE: Do not use the Doc-ItLS software while the camera dial is set to movie mode as doing so may result in an error. Set camera to A-DEP Connect to Doc-ItLS Software 1. Open the Doc-ItLS software.
DigiDoc-It Imaging System 6 System Operation Loading Gels into the Darkroom Hood 1. Turn Off the UV Transilluminator. 2. Open the darkroom door. 3. Place the sample to be imaged in the center of the transilluminator filter glass. 4. Close the darkroom door. 5. Turn On the transilluminator. Note: It is important to minimize the light source exposure to the sample. Turn off the light source as soon as the image is captured. View a Sample Prior to Capture 1. 2.
DigiDoc-It Imaging System 7 4. 5. 6. To take images in color, ensure that the Color Capture box is checked. Select Preview at the top of the software to preview the image. While in preview mode, the Brightness slider can be adjusted to brighten dark images. 7. The user may adjust the focus manually to enhance the appearance of the previewed image. Click the Manual focus radio button on the Preview window, then use the left and right arrows (shown in the red box, below) to adjust the focus settings.
DigiDoc-It Imaging System 2. 8 A new camera window appears with additional options: Camera Control in Manual Mode 3. 4. Select the desired options for an enhanced image. See the camera manual for detailed descriptions of options. Select Capture at the top of the software.
DigiDoc-It Imaging System 9 Service Procedures Return Procedure A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product. Replacement Parts and Accessories Accessories are available for use with the DigiDoc-It Imaging Systems. Contact UVP’s offices at the telephone numbers below for replacement parts for DigiDoc-It equipment.
DigiDoc-It Imaging System 10 Warranty UVP's products are guaranteed to be free of defects in materials, workmanship and manufacture for one (1) year from date of purchase. Consumable and disposable parts including, but not limited to tubes and filters, are guaranteed to be free from defects in manufacture and materials for ninety (90) days from date of purchase. Transilluminators carry a two (2) year warranty from date of purchase.
