ChemiDoc-It® Imaging System Instruction Guide ______________________________________________________________________________ UVP, LLC 2066 W. 11th Street, Upland, CA 91786 Tel: (909) 946-3197 / (800) 452-6788 Fax: (909) 946-3597 Ultra-Violet Products Ltd. Unit 1, Trinity Hall Farm Estate Nuffield Road, Cambridge CB4 1TG UK Tel: +44(0)1223-420022 Fax: +44(0)1223-420561 Web Site: www.uvp.
ChemiDoc-It Imaging System 2 Table of Contents Table of Contents .........................................................................................................................................................2 Introduction ..................................................................................................................................................................3 Components ......................................................................................................
ChemiDoc-It Imaging System 3 Introduction The ChemiDoc-It Imaging System is designed to automate chemiluminescence-based research with onetouch, pre-set or user-defined PC controls for accurate, repeatable imaging and analysis of chemiluminescence and bioluminescence applications. The ChemiDoc-It incorporates a light-tight darkroom ® and VisionWorks LS software to create an ideal, computer-controlled low light imaging environment.
ChemiDoc-It Imaging System 4 Components The ChemiDoc-It Imaging System is comprised of the following equipment: Darkroom Cabinet CCD Camera: o MegaCam 810 o OptiChemi™ 610 o BioChemi™ 510 Lift Platform (manually adjustable) White Epi Illumination Chemi Tray VisionWorksLS Software Refer to the packing slip for a complete equipment list. System components may vary. The system may additionally include a computer and monitor.
ChemiDoc-It Imaging System Setup Instructions Installing VisionWorksLS Software 1. Insert the VisionWorksLS flash drive into the computer. 2. Click on the Install button for VisionWorksLS. 3. Click OK, Next, agree to “I accept terms of licensing agreement”, then Next. Leave all options in their default settings. Then click Next, Next, Install and finally Finish. Registering the Software Open the Software 1. Double click the VisionWorksLS software icon on the desktop. 2.
ChemiDoc-It Imaging System 8. If the computer is not connected to the Internet, click Offline activation to register the software. This allows the user to obtain the activation code and enter it at another time. 9. Click Next to continue. 10. Click the link provided and complete the form to obtain registration instructions. Click Finish. Hardware Installation Darkroom Setup 1. Remove all the tape from the darkroom. 2.
ChemiDoc-It Imaging System 7 Motorized Lens Controller Setup The camera, lens and lens controller are all connected at the factory and come shipped as a single unit. Note: Ignore this section if the system was shipped with a manual lens. Check the packaging slip for additional information about product configuration. UVP Lens Controller (pictured without camera and lens) The lens controller is attached to the lens at the factory.
ChemiDoc-It Imaging System 8 Note: The MegaCam 810, OptiChemi 610 and BioChemi 510 cameras use many of the same components and are installed in a similar manner. Therefore, the installation instructions herein will refer to all three cameras interchangeably. Note: When looking at the darkroom from the front, the MegaCam 810, OptiChemi 610 and BioChemi 510 cameras attach to the bracket with the Black Thumb Screw at the right of the system.
ChemiDoc-It Imaging System 9 Installation with Manual Lens (BioChemi 510 and OptiChemi 610 Only) BioChemi 510 & OptiChemi 610 Components 1. USB Cable 2. Camera 3. Base Mount and Gasket 4. Bracket 5. Black Thumb Screw 6. Step-Up Ring 7. Manual Lens 8. Magnifier 9. Diopter 10. Brass Thumb Nuts 2 3 1 6 10 5 7 8 9 4 11 12 11. Power Cord 12. Camera Power The photograph shows the parts required for assembly of the OptiChemi 610 camera with manual lens on the ChemiDoc-It Imaging System.
ChemiDoc-It Imaging System 10 Software Installation All of the software required to operate the MegaCam 810, OptiChemi 610 and BioChemi 510 cameras, as well as their respective motorized lens controllers (if equipped), is embedded within VisionWorksLS. Therefore, once VisionWorksLS has been installed, plug in the camera and lens controller. No additional drivers or software are required. Thermal Printer Setup If this order did not include a thermal printer, move on to the next section.
ChemiDoc-It Imaging System 11 Operation Using the System The master power switch is located on the upper right side of the darkroom (see picture below). Connect the system power as described in Hardware Installation. 1. Turn on the darkroom Main Power switch. 2. Open VisionWorksLS software. 3. The ChemiDoc-It Imaging System may be configured with a motorized lens that is controlled by VisionWorksLS software. The software has default settings which can be modified with user-defined templates.
ChemiDoc-It Imaging System 12 Capturing Images and Using Templates Focusing the Lens Using VisionWorksLS Preview 1. Place the sample or focus target on the surface where the sample will be imaged. 2. Turn on the darkroom Epi White Light switch (see page 13 for more information). 3. Open VisionWorksLS. Start the image preview by clicking on the Preview button on the main VisionWorksLS screen. With the darkroom door open, move the sample around as necessary. 4.
ChemiDoc-It Imaging System 13 Using Templates Set Up and Save Camera Templates NOTE: Without using templates, camera settings can be manually adjusted directly in the software. Chemi Blot Pre-Capture Positioning: For this application, use a preview binning of 1x1, a capture binning 1x1 and an exposure time of 0.2sec. Have the darkroom door cracked open to let in some ambient light.
ChemiDoc-It Imaging System 14 Service Procedures Return Procedure A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product. Troubleshooting No Power to the Darkroom 1. Recheck main power cord connections to the darkroom. 2. Check fuses, located at the back of the unit, near the power port. A flat-head screwdriver will be required. Turn the fuseholder cap counterclockwise and the fuse holder will pop out.
