GelDoc-It®TS2 & ChemiDoc-It®TS2 Imagers Installation and User Instructions UVP, LLC 2066 W. 11th Street Upland, CA 91786 Phone: (800) 452-6788 Fax: (909) 946-3597 Ultra-Violet Products Ltd. Unit 1, Trinity Hall Farm Estate Nuffield Road, Cambridge CB4 1TG UK Phone: +44(0)1223-420022 Fax: +44(0)1223-420561 Web Site: www.uvp.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 2 Table of Contents Introduction ..................................................................................................................................................................3 System Components ...................................................................................................................................................4 Specifications .....................................................................................
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 3 Introduction TS2 The GelDoc-It Imager is a high resolution imaging system capable of capturing and documenting fluorescent gel images. TS2 In addition to gel documentation capabilities, the ChemiDoc-It Imager is equipped with a cooled, scientific-grade CCD camera allowing users to capture and document chemiluminescent blot images, including Western blots. The TS2 Imagers are self-contained, light-tight imaging systems with a built-in 15.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 4 System Components Refer to the packing slip and pictured components for specific parts and components included with the system. 15.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 5 Specifications Power Requirements: 100/115V, 50/60Hz; 3.1 Amps at 120 Volts 230V, 50/60Hz; 1.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 6 Darkroom The darkroom is light tight to provide optimal imaging conditions.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 7 Optional Equipment UVP offers a variety of optional equipment to support the needs of varying laboratory environments. Refer to Replacement Parts and Accessories at the end of this manual for optional equipment part numbers.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 8 Converter Plates An alternate to the LED White Light Plate, the UV/White Converter Plate allows imaging of non-fluorescent stained media with an ultraviolet transilluminator. The converter plate is specially coated to convert 302nm UV to white light rather than using a separate white light box.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 9 Setup Instructions Components TS2 When unpacking the GelDoc-It 1. 2. 3. 4. 5. 6. 7. 8.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 10 Installing Emission Filters 2 To install the 50mm ethidium bromide (EtBr) filter and any other emission filters: 1. Carefully remove the filter from the protective plastic case, holding the filter at the edges to prevent placing fingerprints on the glass surface. 2. The filter wheel is located on the user’s right side of the darkroom. Press in on the slide-out filter wheel door and the door will pop out slightly.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 11 Using the System Powering Up the System Set the black power switch on the back of the system to the ON (I) position. Once plugged in to a wall outlet and the black power switch on the back of the system is placed in the ON (I) position, the TS2 TS2 GelDoc-It and ChemiDoc-It systems are always powered on. Press the POWER button on the front of the Power Indicator system to power on the internal Lights computer/touch screen.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 12 Operating the TS2 Software Interface Upon startup, the internal computer will proceed through the boot-up process. When complete, the Windows desktop will appear. The TS2 Software screen, similar to the one below, will open automatically shortly thereafter. To exit the TS2 Software interface, press either the close (X) or minimize (_) buttons at the top right corner of the software (see red circle above).
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 13 Image Zooming TS2 TS2 Both the GelDoc-It with the GelCam 310 camera and the ChiemiDoc-It with the BioChemi 510 camera are equipped with optical zoom lenses, meaning that the system uses the lens’ optics to make the sample appear closer/larger on the screen. Optical zoom is adjusted using the vertical slider in the Zoom menu on the TS2 Software screen.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 14 Touch Screen Interface Setting User Preferences The Settings portion of the TS2 Software allows the user to select preferences which are normally set once and rarely changed. Such settings include image save format and image save location.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 15 Auto Invert Image: Set Auto Invert Image to ON to automatically invert the image upon image capture. Note: “Auto Invert Image” must be set to ON in order to invert images during image capture; captured images can also be inverted in the Gallery view. Noise Subtraction: Set Noise Subtraction to ON to reduce the amount of background and ambient (“white”) noise within the image. In most circumstances, this setting should be left ON.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 16 Changing Lighting and Filter Settings Before previewing or capturing images, choose the appropriate lighting and emission filter settings. Lighting Press the Lighting button on the main TS2 screen to access the lighting selection menu. The following options will appear: Epi Illumination: Press the desired radio button to select from the following epi illumination sources: White, Accessory or Off. 1.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 17 Edit: To change a filter name, press the desired radio button to select the emission filter and then press the Edit button. A screen will appear allowing the user to type in a new filter name. Press the Checkmark button to accept the new name or the “X” button to cancel the filter name change.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 18 Save After Capture function is enabled in Preferences, the image will automatically be saved to the preset location (see “Saving Tab” under Setting User Preferences in this manual). Use the slider bar to adjust various settings, including Exposure, Aperture, Histogram, Focus and Zoom (if applicable).
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 19 • Manual Exposure: Captures one (or more) images with a pre-selected exposure time. • Auto Exposure: Captures an image with an ideal exposure time determined automatically by the system prior to image capture. (NOTE: When using the Auto Exposure function, any exposure time settings made by the user prior to image capture will be changed.) After selecting Auto Exposure, select from one of the following image acquisition settings: 1.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 20 Press the Histogram button to access image histogram adjustment. Use the pointers on the vertical slider bar to make adjustments to this setting. Slide the top marker down to darken the image, or slide the bottom marker up to lighten the image. Push the “Reset” button to reset the markers to the top and bottom of the slider bar, showing the image’s full histogram range.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 21 Turn on Saturation Warning to provide a bright yellow or red image overlay on oversaturated areas of the image during Live Preview. Yellow indicates mild overexposure while red indicates extreme overexposure. Saturation Warning To capture an ideally exposed image, decrease the aperture or exposure time until the yellow or red overlay disappears. To activate saturation warning, press the Saturation Warning slider until the check mark appears.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 22 Use the Gallery Navigation buttons to navigate through the image gallery. When multiple “pages” of images appear in the gallery: • Press Previous to go to the previous page. • Press Next to go to the next page. Gallery Navigation • Press Last to go to the newest picture in the Gallery. • Press Active to go to the active image shown on the main image screen.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 23 Press the Save Burned button to save the image with all modifications (such as time stamp and histogram modifications) embedded in the image. Or, press the Save button to save the raw image without any modifications embedded. Images will be saved to the location specified in Preferences.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 24 To access image compositing tools, press to select the Compositing tab. “Compositing” Tab Copositing tools include Fluorescent, Merge and Chemiluminescent. Press to select the desired compositing tool once the Compositing tab has been selected. The Fluorescent Compositing Tool is used to merge various fluorescently-colored channels into one image. To use this tool, first open two or three images in the gray area (gallery bar) below the “Compositing” tab.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 25 Once the ideal composited image has been created, press the Checkmark button below the Preview composited image to complete fluorescent compositing. The Chemiluminescent Compositing Tool is used to merge various image channels for chemiluminescent imaging. Chemiluminescent Compositing Tool This tool functions exactly the same as the Fluorescent Compositing Tool described earlier, except that the middle overlay image will always automatically be inverted.
TS2 GelDoc-It TS2 and ChemiDoc-It 4. Imagers 26 To select the quick-access templates to be shown at the top of the TS2 screen, press the Templates button until the list of saved templates is shown. Then, drag the template to the desired quick-access position. To run a template, either: 1. Select the desired template from the Templates menu by pressing the gray check box icon to the right of the template name, or 2. Select the template from the quick-access area.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 27 Service Procedures Return Procedure A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product. Replacement Parts and Accessories To order accessories or replacement parts for the GelDoc-It offices. TS2 TS2 and ChemiDoc-It Imagers, contact UVP’s Part Description Part Number Epi UV Modules: UV Module, 254/365nm, 4 watt (115V) UV Module, 254/365nm, 4 watt (230V) 95-0021-12 (Qty.
TS2 GelDoc-It TS2 and ChemiDoc-It Imagers 28 Inspect the thin wire within each glass fuse to see if there is a break in the wire. If so, replace the fuse(s). If fuses are blowing repeatedly, contact UVP Technical Support for additional troubleshooting. Transilluminator Will Not Turn On 1. In addition to turning on “UV transillumination” in the Lighting menu within the TS2 Software, the transilluminator itself has a power switch.
