Instruction manual

Fluid Operation
Lysozyme on Mica—A Model Procedure for Protein Binding
144 MultiMode SPM Instruction Manual Rev. B
9. After the fluid cell has been flushed with buffer solution, reclamp the drain line. This is
important for low-noise, low drift imaging. The sample is now ready for TappingMode
imaging. A good TappingMode image of lysozyme protein on mica is shown in Figure 8.6b
Figure 8.6b TappingMode image of lysozyme in buffer solution using above sample preparation (Scan size =
500nm).
Note: It is also possible to prepare samples inside the fluid cell by flowing the protein
solution through the fluid cell. In this case, it may be helpful to engage the tip in
contact mode with a zero Scan size to protect proteins from binding to the tip.