Instruction manual

List of Figures
Rev. B MultiMode SPM Instruction Manual 15
Chapter 7 TappingMode AFM. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .103
Figure 7.1a Tapping Cantilever in Free Air . . . . . . . . . . . . . . . . . . . . . . . . .104
Figure 7.1b Tapping cantilever on sample surface. Note deflection of cantilever and
return signal (exaggerated).. . . . . . . . . . . . . . . . . . . . . .104
Figure 7.2a Select Show All Items . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
Figure 7.2b Enable Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
Figure 7.2c Photodetector adjustment knobs and SPM voltage meters. . . .107
Figure 7.2d Voltage meters on the MultiMode base reveal a great deal about the
amplitude and alignment of the TappingMode laser signal on a
tuned tip operating at its resonant frequency. . . . . . . . .108
Figure 7.2e Cantilever Tune Panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .110
Figure 7.2f Sweep control panels for Graph Controls (left) and Main Controls
(right). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .111
Figure 7.3a Suggested Scan Controls Settings During TappingMode Setup113
Figure 7.3b Suggested Other Controls Settings During TappingMode Setup114
Figure 7.5a Cantilever Response Curve . . . . . . . . . . . . . . . . . . . . . . . . . . . .115
Figure 7.5b Scope Trace with High Scan Rate. . . . . . . . . . . . . . . . . . . . . . .116
Figure 7.5c Scope trace with Correct Scan Rate . . . . . . . . . . . . . . . . . . . . .117
Figure 7.5d Suggested Range for Drive Frequency . . . . . . . . . . . . . . . . . . .117
Chapter 8 Fluid Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
Figure 8.2a Fluid Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124
Figure 8.2b Load Probe into Fluid Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . .127
Figure 8.2c Fill the Fluid Cell with Liquid . . . . . . . . . . . . . . . . . . . . . . . . .128
Figure 8.2d Stainless Steel Sample Puck with Teflon Cover . . . . . . . . . . . .130
Figure 8.2e Flush the Fluid Cell Before Installation to Reduce Bubble Formation
130
Figure 8.2f Imaging a Sample Covered by a Drop of Fluid. . . . . . . . . . . . .131
Figure 8.3a A 100µm, Narrow-legged, Si
3
N
4
Cantilever Fluid Tune Curve 135
Figure 8.6a Proteins will typically bind to negatively charged mica when the pH is
reduced below the protein’s isoelectric point, pI . . . . .142
Figure 8.6b TappingMode image of lysozyme in buffer solution using above
sample preparation (Scan size = 500nm).. . . . . . . . . . . 144
Figure 8.7a Negatively charged DNA may be bound to negatively charged mica in
the presence of divalent counterions, such as Ni
+2
. . . .145
Chapter 9 Scanning Tunneling Microscopy (STM) . . . . . . . . . . . . . . . . . . . . .147
Figure 9.1a STM Converter Head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .149
Figure 9.2a Select Show All Items . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .152
Figure 9.2b Enable Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .152
Figure 9.5a Low-Current Converter components: MultiMode head and Picoamp
Boost Box.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .157
Figure 9.5b STM current and height images of HOPG surface.
Scan size = 6.0nm, Itun = 1.6pA, Vbias = 29mV.. . . . .161