Instruction manual

Rev. B MultiMode SPM Instruction Manual 121

Chapter 8 Fluid Operation

This chapter addresses scanning probe microscope (SPM) imaging of samples in ﬂuid using a

MultiMode. Refer to the following for your speciﬁc area of interest:

• Introduction: Section 8.1

• General Fluid Operation: Section 8.2

• Clean Fluid Cell and O-ring: Section 8.2.1

• Select the Probe: Section 8.2.2

• Remove Organic Contamination from the Tip: Section 8.2.3

• Load the Fluid Cell with a Probe: Section 8.2.4

• Sample Mounting: Section 8.2.5

• Align the Laser: Section 8.2.6

• Adjust the Detector Offsets and Setpoint (Contact Mode): Section 8.2.7

• Engage the Surface: Section 8.2.8

• Adjust Scan Parameters (Contact Mode): Section 8.2.9

• Clean and Dry Parts When Done: Section 8.2.10

• TappingMode in Fluids: Section 8.3

• Procedure for TappingMode Imaging in Fluid: Section 8.3.1

• Plotting Amplitude vs. Distance (Force Cal Curve): Section 8.3.2

• Optimizing Image Quality: Section 8.3.3

• Troubleshooting Tips: Section 8.4

• Cantilever Tune Plot Looks Poor: Loose Probetip: Section 8.4.1

• Laser Sum Signal Absent or Weak: Air Bubbles: Section 8.4.2