User`s guide

4-40
Operation 4
Example 2
The operation of the method in Example 2
will be as follows.
File # 4 contains the method program. The
flow rate remains constant throughout the
program. At 0.00 minutes the mobile phase
will have a constant flow rate of 3 mL/min
and a composition of 95% A, 5% B and 0%
C. The composition will change to 70% A,
30% B and 0% C in a time of 1 minute. The
composition will stay constant for 1
minute and then return to 95% A, 5% B
and 0% C in a time of 0.5 minutes. The
composition will remain at 5% B until 4.00
minutes. The program will loop and repeat
the same method 3 times and will then
link to File 5.
File 5 will contain a program to set the flow rate to
0 mL/min. The Low pressure and High pressure
safety files that were used in the first example can
also be used for this example. It is not necessary to
reprogram them.
Enter this program by following the steps given in
Program list 4 and 5.
The system is now ready to run. Press
Run.The display will look as opposite.
Press Roll to see the % of A and B changing.
After 2.5 minutes, the program will loop to
start the method a second time. The Loop
Number will change to 2.
At the end of three loops, the program will
link to File 5. File # 5 is displayed. At the
end of File # 5 the Display will go back to
File # 4. Note that in this program, File # 5
only lasts for a short time.
Programming Examples