User`s guide

4-35
Operation 4
Press Run to start the conditioning of the column.
The pump will ramp to the flow rate and solvent
composition that is programmed in the time pro-
grammed. The values for time, flow rate and
solvent composition can be changed during the
conditioning. At the end of the ramp time, the flow
rate and solvent composition will remain constant.
Note that you can leave the conditioning of the
column and create a program without interfering
with the conditioning of the column.
After pressing Run, the display changes to give you
the choice of Roll, Stop or Quit.
Pressing Roll displays in turn the time for the ramp,
the flow rate, the proportion of solvent A, B and C
in turn. The proportion of solvents B and C will not
appear if they do not exist.
This part of the software is also used at the end of a
method program to clean the column. The initial
conditions will be the final flow rate and composi-
tion which existed at the end of your method
program. You can ramp the flow rate down to 0
mL/min and create a solvent gradient that will
completely clean the column.
The Quit soft key brings you to the Select Menu
Item menu. This gives you access to the pump
setup parameters, the pump I/O parameters and
the method file. Press this key to verify or change
the value of any parameters in these sections.
Run Soft Key
It starts the program running. When this key is
pressed, the display changes. The figure in the top
left hand corner is the file number. The number in
parentheses is the loop number. If there is more than
one loop in your program, the present loop number
will be displayed here. The first time displayed is
the actual running time. The second time displayed
is the total running time for the method program.
When a program is written, the current values for
the six parameters Pump Head Size, Compressibility,
Refill time, Inlet Pressure, High Pressure Limit and
Low Pressure Limit are stored as part of the
method program.
Running a Method Program