User`s guide

4-34
Operation 4
Running a Method Program
At the end of programming the method and error
files, the software returns to a Ready-to-Run
Screen. The top left corner will indicate which
Ready-to-Run screen you are in. To go to the
Program mode Ready-to-Run Screen:
• press Menu.
• press Mode.
• press Prog.
The pressure can be displayed in 3 units, bar, MPa
or kpsi. To change from one unit to another, press
the soft key directly below bar. The units will
change to MPa. Pressing the soft key again will
change the units to kpsi. Pressing the soft key again
will change the units back to bar.
You can go to any file by keying in a new number
and pressing Enter. The Ready-to-Run Screen
displays the present file number, the pressure, and
gives you 3 soft key options, Cond, Menu and Run.
Cond Soft Key
It brings you to the part of the software which is
used to condition the column. You program a ramp
time, the flow rate and gradient. The initial condi-
tions are the existing conditions for the pump, i.e. for
a pump which is not running 0 mL/min and 100%
solvent A. The flow rate and composition program-
med should be the flow rate and composition that
is required at the start of the method program that
you are going to use. The solvent flow rate and
composition are then ramped from 0 mL/min and
100% A to the initial composition and flow rate for
your method program.
• press Cond.
• enter the ramping time and press Enter.
• press Flow.
• enter the flow rate for the end of the ramp time.
• enter the % B for the end of the ramp time (if
pump B is present).
• enter the % C for the end of the ramp time (if
pump C is present).
Running a Method Program