User`s guide

4-30
Operation 4
At the selected time, the Master 305 starts the
injection pump and automatically lowers the
elution rate in order to keep the total flow rate, and
hence the pressure, constant. The following rela-
tionship is applied:
F’
e
= F
e
- F
i
where
F’
e
: the total elution flow rate during injection
F
e
: the total elution flow rate before injection
F
i
: the injection flow rate
A stop-flow injection, generally desired, is then
obtained by selecting Fi 암 Fe.
Reading/Writing/Editing Timed Events
The five different types of timed event have been
explained above. At the end of each event, the
menu gives you five options. These options are
explained below.
Next: displays the next timed event in the se-
quence. If there are no more events, it dis-
plays End of file.
Prev: displays the previous timed event in the
sequence. If there are no more previous
events, it displays Beginning of file.
Add: brings you to the Choose an event type
menu to allow you to add another event to
the present program.
Del: deletes one timed event in the program. As
a safeguard it asks you, Delete this point ?
Press Yes to delete the event or No to return
to the original menu.
End: leaves the software for programming timed
events. The pump displays Solvent con-
sumption in mL for pump A for one com-
plete run of the method program.
Pressing the Next soft key displays in turn the
consumption for the other pumps in the system.
The minimum consumption is < 0.1 mL. The maxi-
mum is > 999 mL.
Press Quit to go to the Ready-to-Run Screen. You
have now finished creating a program method file.
Before running a method program, you should
program each of the four safety/error files.
Programming a Method