User`s guide

4-14
Operation 4
• Set the GSIOC ID number for the 305 Master pump.
• Program outputs to be Open/Closed.
• Set pause in program to be with or without flow .
• Select the Gradient profile output to be Flow, %B,
or %C.
• Specify the Delay Volume. (Maximum value 999 mL)
• Zero the Manometric Module.
• When all I/O functions have been defined as
required, press the Quit soft key.
Setting Up the Pump
After entering the data about the pumping system, the
pump is ready to run. The 305 Master pump can
operate in three different modes. These modes are:
Flow: The 305 pump provides a constant flow rate. The
pump starts when the Run key is pressed and stops
when the Stop key is pressed. The flow mode is for
isocratic use only.
Dispense: The 305 dispenses a specified volume. The
pump starts when the Start key is pressed and stops
when the specified volume has been dispensed. The
dispense mode is for isocratic use only.
Program: The 305 controls a multi-pump system with
up to 2 slave elution pumps and 1 slave injection
pump. In this mode, the 305 Master pump can create
gradients of flow rate and composition, open and close
outputs to control other instruments and wait for
signals from other instruments.
The operation of each mode is explained.