User`s guide

4-5
Operation 4
With a new pumping system, you must enter data
about each pump connected, i.e. Refill Time, solvent
Compressibility and pump Head Size and Inlet
Pressure. This is done using the Pump branch of the
software. Data concerning the overall pumping
system, for example high pressure limit, low pressure
limit etc. is done using the I/O branch of the software.
The File branch is used for writing files of method
programs. The Mode branch is used to operate the
system in one of three possible modes, Flow,
Dispense, or Program Mode.
The specifications for Pump and I/O must be set for
a new system setup, or when the physical setup of
the pumping system is changed, for example,
changing a pump head size or removing or intro-
ducing a pump.
Set Up Pump Hardware (PUMP)
A pumping system consists of a minimum of 1
pump and a maximum of 3 elution pumps and
1 injection pump. Pump A, the first elution pump
(the 305 Master pump), is always present.
The sequence of screens and soft key command
options for the setup of pump hardware is shown
in page 4-6.
To go to the pump setup menu:
• press Menu
• press Pump
Use this menu to enter data about pump A.
• Select pump A by pressing the soft key below A
• The sequence for defining parameters is:
1. Refill time
2. Liquid Compressibility
3. Head size
4. Inlet Pressure (for liquefied gas)
A value for each parameter is keyed in using the
keypad and is stored in memory by pressing
Enter. This automatically brings you to the next
menu screen. If you do not want to change the
value already stored in memory, press the Next
soft key or press Enter.
Setting Up the Pump
The maximum flow rate
depends on the Head Size and
the refill time. If the refill time
is too long, a message Invalid
settings flashes when you run
the program. The refill time or
flow rate must be lowered. The
figure below shows a curve of
refill times versus flow rate.