User`s guide

4-2
Operation 4
Priming the Pump Head
Priming the Pump Head
Do not run the pump when the pump head is dry.
This can result in severe pump head damage.
Check that the solvent bottle is filled with HPLC
grade, degassed solvent or buffer. Immerse the inlet
tubing filter into the solvent reservoir. Make sure that
all of the hydraulic connections are properly made.
Before priming, all electrical connections must be
made, and all hydraulic connections in place. All
pumps present in the system, A, B, C and Inj can
be primed in the Program Mode using the 305
Prime command. However, the pumps must be
connected and declared present in the “Setup
Pump Hardware” procedure, page 4-5.
For 5SC, 10SC, 10WSC and 10WTi pump heads,
use the syringe supplied with the pump head to
prime the pump as follows:
• Attach the syringe to the luer fitting of the low
pressure prime valve (refer to the figure below).
• Draw liquid into the syringe with the low pressure
prime valve in the SYRINGE LOAD position.
• Turn the valve to the SYRINGE-INJECT position.
Press the PRIME key on the front panel of the
305, the screen will indicate which pumps are
present, and you simply select which pump you
wish to prime by pressing the Pump key until the
desired pump has been selected. Press Run and
the pump will start running at its maximum speed.
Depress the syringe (if used) until the pump inlet
is clear of bubbles and some liquid has passed
through the pump outlet.
• Turn the valve to the RUN position.
Remove the syringe from the prime
valve. When no bubbles can be seen
at the outlet tubing, press the STOP
key to end the priming procedure.
Check that there are no leaks in
the system.
For the 25SC, 50SC, 100SC, 25WTi
and 200WTi pump heads,
• prime the pump directly without
syringe or valve.
Syringe
Inject position
Syringe
Load position
Pump Inlet
Inlet filter
10 µm
Run position
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