Operating instructions

4. Calibration page 38
Mercury Instruments UT-3000-E_V2.11.docx 31.07.12
Analytical Technologies
e) Purge the syringe with calibration gas by pulling and pushing the plunger slowly a few
times. Then pull the plunger a little bit further than it is necessary to get the desired
volume and pull the syringe vertically out from the septum.
f) Move the plunger exactly to the desired volume and push the tip of the syringe capillary
into the septum of the of the calibration port on the front panel of the UT-3000. Push it all
the way as far as possible to make sure the tip is at the correct position. Try to center the
capillary so that it can penetrate the calibration port without getting stuck. Inject the
calibration gas by pushing the plunger of the syringe slowly. Then pull the capillary out of
the calibration port and put the syringe down.
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 CAUTION: Contamination of parts of the syringe or the calibration port of the UT-
3000 with liquid mercury has strictly to be avoided ! Even smallest droplets of
mercury which cling to the syringe capillary or the septum of either the calibration
device or the calibration port will cause a severe contamination. Indication for a
contamination are: too high absorbance values of calibration points, erratically
scattered calibration points, unexpected high measurement peaks. In this case all
the equipment which may have been contaminated has to be exchanged, including
septa, syringe capillary or even the whole syringe if contaminated inside.
Figure: use closed cap for transportation (left) and septum cap for calibration (right)
Performing a manual calibration using the Static Calibration:
1. Make sure a new septum has been inserted in the calibration port of the instrument.
2. Check if for Cal. Mode X= aX is selected (recommended), now press F2- Enter µl in the
Manual Calibration menu.