User`s guide

TO SET UP/EDIT AN AUTOFILL FLOW PROFILE:
1. From the Step Definition screen for an Autofill profile, choose the Set mode button to toggle between
the Withdraw/Infuse (WD/INF) and Infuse/Withdraw (INF/WD) modes. Withdraw/Infuse begins by
withdrawing into the syringe, following by infusing into the target.
2. Choose the Set Rates button to enter the Set Infuse/Withdraw Rate screen.
3. Select the Infuse Rate (it should be selected by default as indicated by the active data entry box to the
right of the button). Enter the desired rate using the keypad or select the Min Rate or Max Rate
button.
4. If the Units of Rate are not correct for your application, choose the Units button.
5. On the Units Selection screen, select the unit of measure (ml, µl, nl or pl) and the unit of time (sec,
m
in, hr) as appropriate, then choose Accept to confirm your selections.
6. Select the Withdraw Rate and enter the desired rate using the keypad or select the Min Rate or Max
Rate button.
7. When all parameters are correctly set on the Set Infuse/Withdraw Rate screen, choose Accept to
confirm your settings and return to Step Definition screen.
8. Choose the Set Volume per Cycle button to enter the Set Volume per Cycle screen.
9. Enter the Volume using the keypad.
10. If the Units of Measure associated with Volume are not correct for your application, choose the Units
button to enter the Units selection screen. Select the unit of measure button (ml, µl, nl, pl) appropriate
for your application, then choose the Accept button to return to the Set Target Volumes screen.
11. When all parameters on the Set Volume per Cycle screen are correct, choose the Accept button to
return to the Step Definition screen.
12. Choose the Set Total Volume or # Cycles button to enter the Set Total Volume or # Cycles screen.
13. Select either Total Volume or # Cycles to define how the pump will control pumping activity. Total
Volume will cycle the pump the correct number of times to deliver the total volume according to the
Volume per Cycle setting. For example, if you set a 10 ml Volume per Cycle and set 100 ml in Total
Volume, the pump will cycle 10 times.
14. If you select Total Volume, enter the volume desired using the keypad. If you select # Cycles, enter the
total number of Infuse/Withdraw cycles that you wish to perform.
15. If the Units of Measure associated with Volume are not correct for your application, choose the Units
button to enter the Units selection screen. Select the unit of measure button (ml, µl, nl, pl) appropriate
for your application, then choose the Accept button to return to the Set Target Volumes screen.
16. When all parameters on the Set Total Volume or # Cycles screen are correct, choose the Accept button
to return to the Step Definition screen.
✐
NOTE: Using maximum rate may not be appropriate during the Withdraw stage of an
Autofill profile, since air can be drawn into the syringe, or a high vacuum pressure can be
produced depending on the syringe, tubing being used, or the fluid viscosity.
Harvard Apparatus PHD ULTRA
™
Syringe Pump Series User's Manual
Publication 5419-002-Rev-A
67
U
sing The PHD ULTRA™