User`s guide

TO SET UP/EDIT A PULSE RATE PROFILE:
1. From the Step Definition screen for a Pulse Rate profile, choose the Set Mode button to toggle the
Flow Mode between Infuse and Withdraw operation.
2. Choose the Set Rates button to enter the Set Pulse Rates screen.
3. Select the Rate 1 button (it should be selected by default as indicated by the active data entry box to
the right of the button). Enter the flow rate value desired using the keypad or select the Minimum
Rate or Maximum Rate button.
4. If the Units of Rate are not correct for your application, choose the Units button.
5. On the Units Selection screen, select the unit of measure (ml, µl, nl or pl) and the unit of time (sec,
min, hr) as appropriate, then choose Accept to confirm your selections.
6
. Repeat Steps 3 to 5 for the second flow rate by selecting the Rate 2 button and continuing data entry.
7. When all parameters are correctly set on the Set Pulse Rates screen, choose Accept to confirm your
settings and return to Step Definition screen.
8. Select the Volume or Time button to determine how the pump cycle length will be managed. Volume
calculates each cycle stage by pumping for the length of time needed to pump the specified volume.
Time manages each cycle stage by pumping for the amount of time specified.
9. Depending on whether you have selected the Volume or Time button in the preceding step, choose
the Set Time or the Set Volume button to enter the Set Pulse Times or the Set Pulse Volumes screens.
Figure 55: Set Pulse Times screen used to define the length of time the pump will operate at each pulse step level.
10. If you selected Set Time, continue with Step 11. If you selected Set Volume, proceed to Step 14.
11. On the Set Pulse Times screen, select the Time 1 button (it should be selected by default as indicated
by the active data entry box to the right of the button). Enter the desired value using the keypad.
Repeat for Time 2.
12. If the Units of Time associated with Time 1 or Time 2 are not correct for your application, select the
Units button associated with either parameter to toggle between sec (seconds) and h:m:s
(hours:minutes:seconds). To use the h:m:s option, enter your time value in hours, minutes and
seconds, separating each value with a colon.
13. When both Time parameters are set to the correct values, choose the Accept button to return to the
Step Definition screen.
14. On the Set Pulse Volumes screen, select the Volume 1 button (it should be selected by default as
indicated by the active data entry box to the right of the button). Enter the desired value using the
keypad. Repeat for Volume 2.
15. If the Units of Volume associated with Volume 1 or Volume 2 are not correct for your application,
choose the Units button associated with either parameter to enter the Units selection screen. Select
the unit of measure button (ml, µl, nl, pl) applicable to your application, then choose the Accept
button to return to the Set Pulse Volumes screen.
16. When both Volume parameters are set to the correct values, choose the Accept button to return to the
Step Definition screen.
17. Select the # of Pulses button to enter the Set # Pulses screen.
18. On the Set # Pulses screen, enter the desired value using the keypad, then choose the Accept button.
Harvard Apparatus PHD ULTRA
Syringe Pump Series User's Manual
Publication 5419-002-Rev-A
60
U
sing The PHD ULTRA™