PHD ULTRA™ Syringe Pump Series User's Guide PHD ULTRA™ Series MA1 70-30xx PATENTS PENDING Publication 5419-002-REV-A
Table of Contents Table of Contents SUBJECT PAGE NO. Quick Guide........................................................................................................................10 10 Things You Need To Know To Use The PHD ULTRA™ ......................................................10 General Information ........................................................................................................11 Conventions Used In This Guide .........................................................
Table of Contents Maintenance/Calibration Reminders ..........................................................................................28 Power Up Running ........................................................................................................................29 Syringe Loading ..................................................................................................................................30 Changing the bracket for small (<30ml) vs large (>30ml) syringes: ..........
Table of Contents Concentration Profile ................................................................................................................63 Autofill Profile ............................................................................................................................66 Incorporating Advanced Options (Commands and I/O) Into A Method ..........................68 Go To Step .........................................................................................................
Table of Contents Appendices ........................................................................................................................89 Appendix A: Syringe Volume/Diameter Reference Table ..........................................................89 Appendix B: Stainless Steel Syringes ..............................................................................................90 Appendix C: Min/Max Flow Rates ...............................................................................
Table of Contents LIST OF FIGURES Figure 1: PAGE NO. Rear view of the PHD ULTRA™ pump showing important connections. ..........16 Figure 2: Front view of the PHD ULTRA™ pump with important components and controls. 16 Figure 4: The PHD ULTRA™ Touchscreen Display. The screen shown is the Quick Start display. ..............................................................................................................................
Table of Contents Figure 27: Method Selection screen showing a listing of Methods in an Infuse/ Withdraw Programmable model PHD ULTRA™ pump. ........................................37 Figure 28: Quick Start Run screen for a Withdraw/Infuse Method. ........................................38 Figure 29: Method Run screen for Preloaded and User-Defined Methods. ............................38 Figure 30: Quick Start Method Run screen after a Method has been started. ........................
Table of Contents Figure 59: Step Definition screen for a Concentration profile. ................................................63 Figure 60: Set Animal weight screen in Concentration profile. ................................................64 Figure 61. Set Rate and Concentration screen in Concentration profile. ..............................64 Figure 62: Set # Doses and Time Lag screen in Concentration profile. ................................65 Figure 63: Autofill Flow Pump Operation Profile. ......
Table of Contents Figure 93: Daisy-Chaining with Legacy RJ-11 Option. ............................................................108 Figure 94: RS-485 In/Out - IEEE-1394 Sockets ......................................................................109 Figure 95: Daisy -Chaining with RS-485. ....................................................................................109 Figure 96: 14-POS, Circular Plastic Female AMP P/N 206043-1 ..........................................
Quick Guide Quick Guide 10 THINGS YOU NEED TO KNOW TO USE THE PHD ULTRA™ Since many of our users are already familiar with the operation of a syringe pump, we’ve created this onepage summary of important tips for using the PHD Ultra. Operations Display: this section of the screen is used to display pump operations, method information, general parameter settings and the real-time display of data while the pump is running.
General Information General Information CONVENTIONS USED IN THIS GUIDE This guide describes basic procedures for operating the PHD ULTRA™ Syringe Pump, including both hardware operations and software operations accessed through the pump’s touchscreen display.This section discusses important conventions used in this guide. Capitalization All menus, commands, and dialog box option names appear with initial capital letters whether or not they are completely capitalized in the user interface.
General Information WARRANTY AND REPAIR INFORMATION ! CAUTION: REFER TO SAFETY INFORMATION AND SETTING UP THE PHD ULTRA BEFORE PLUGGING IN PHD ULTRA™ PUMP. Manual Description This manual is designed to provide all operational and program information required to operate and maintain the PHD ULTRA™ series pumps.The functions and features are described in the Technical Specifications section. Warranty Harvard Apparatus warranties this instrument for a period of two years from date of purchase.
General Information SAFETY INFORMATION Please read the following safety precautions to ensure proper use of your syringe pump. If the equipment is used in a manner not specified, the protection provided by the equipment may be impaired. To Prevent Hazard or Injury: Use Proper Line Cord Use only the specified line cord for this product and make sure line cord is certified for country of use.The operating voltage range for the PHD ULTRA™ is 100-240 vac, 50/60 Hz.
Introduction To The PHD ULTRA™ Introduction To The PHD ULTRA™ Pump PRODUCT OVERVIEW – THEORY OF OPERATION The PHD ULTRA™ Syringe Pump series is a family of high-accuracy, microliter- and milliliter-compatible pumps designed for versatile technical use including mass spectroscopy, calibration, drug and nutritional infusions, microdialysis, dispensing, chromatography and LC/HPLC.The PHD ULTRA™ incorporates a microprocessor controlled, small step angle stepping motor that drives a lead screw and Pusher Block.
Introduction To The PHD ULTRA™ SPECIFICATIONS PHD ULTRA™ Specifications Accuracy ± 0.35% Reproducibility ± 0.05% Syringes (Min./Max.) 0.5 µl / 140 ml Flow Rate: Minimum (0.5 µl syringe) 1.56 pl/min Maximum (140 ml syringe) 220.97 ml/min Display 4.
Setting Up The PHD ULTRA™ Setting Up The PHD ULTRA™ With EZ PRO™ PHYSICAL VIEWS The following diagrams show the important components of the PHD ULTRA™ pump.
Setting Up The PHD ULTRA™ NAVIGATING THE PHD ULTRA™ EZ PRO™ SOFTWARE The PHD ULTRA™ uses a high resolution touchscreen with an icon-enabled graphical user interface and an advanced methods architecture to streamline simple to complex tasks.
Setting Up The PHD ULTRA™ The PHD ULTRA™ supports touchscreen orientation auto-rotation (landscape/portrait) for permitting easy use of the pump when positioned upright on its side. This configuration and the associated touchscreen display are shown in the following figure. Figure 5: The PHD ULTRA™ pump shown in its vertical (upright) orientation and the associated Quick Start menu screen showing the application auto-rotated into portrait mode.
Setting Up The PHD ULTRA™ IN FORMATION/WARNING MESSAGES The Message Area of the touchscreen is used to display helpful instructions for the currently displayed screen. It is also used to display error or warning messages to indicate problem conditions in a Method or error conditions during pump operation.The Message Area is displayed in yellow when one of these warning messages is displayed.
Setting Up The PHD ULTRA™ EZ PRO Touchscreen Button Reference The following buttons are part of the PHD ULTRA™ software: S et t in gs: Access the settings menu used to adjust the following parameters: Date and Time, Audible Alarms, Pusher Block Force, Backlighting, RS-232 Communications, etc. P ag e Do wn: Used to page down in a display list. F a st F or wa rd: Advances the Pusher Block in the forward (infuse) direction. S cro ll Up: Used to scroll up in a display list.
Setting Up The PHD ULTRA™ SYSTEM SETTING ADJUSTMENTS The PHD ULTRA™ Settings menu is used to control basic system operations.This section describes the options available in this section of the application software. . Figure 8: The Settings screen. Setting the Audible Alarms The PHD ULTRA™ pump incorporates an Audible Alarm feature that can be used to warn the operator about various operating conditions, errors and power outages.
Setting Up The PHD ULTRA™ TO SET THE USER-CONFIGURABLE AUDIBLE ALARMS: 1. In the Audible Alarms menu, select the specific alarm or alarms that you wish to set. On the Settings screen, select Touchscreen Lock Enable. Note that a checkbox on the button will be checked to indicate that you have selected an option, but the button will not be displayed in magenta as with other “select” options in the software. 2.
Setting Up The PHD ULTRA™ Locking The Touchscreen The PHD ULTRA™ incorporates a Touchscreen Lock function that can be enabled to prevent accidental operation or interruption of operation.The Touchscreen Lock function makes all buttons on the Method Run display inactive except for the Stop Method buttons. TO ENABLE THE TOUCHSCREEN LOCK FUNCTION: 1. From the Method Main screen, choose the Settings button. 2. On the Settings screen, select Touchscreen Lock Enable.
Setting Up The PHD ULTRA™ TURNING TOUCHSCREEN LOCK OFF To turn Touchscreen Lock off while running a Method, follow these steps: 1. From the Method Run display, the Touchscreen Lock button should be in the Locked mode which appears as a “closed” lock. 2. Select the Method Lock button to cancel Touchscreen Lock.The application will display a warning message in the Message Area asking you to confirm your Unlock selection. 3. Select the Method Lock button again to confirm the unlocking request.
Setting Up The PHD ULTRA™ Setting the Time and Date The PHD ULTRA™ displays the date and time on each screen in the user interface. Built-in battery backups maintain the date and time even if the pump loses power or is unplugged for a period of time. If it is necessary to set the date and time values, follow the instructions below. TO SET THE DATE AND TIME DISPLAY: 1. From the Quick Start or Method Main screen, choose the Settings button. 2.
Setting Up The PHD ULTRA™ Setting Backlight Level You can adjust the backlighting level on the PHD Ultra touchscreen to optimize viewing in your specific lighting conditions. On the Settings screen, the Backlight button displays the current setting on the button. Figure 15: Set Backlight Level screen. TO MODIFY THE BACKLIGHT LEVEL: 1. From the Quick Start or Method Main screen, choose the Settings button. 2. From the Settings menu, choose the Backlight button to enter the Set Backlight Level screen.
Setting Up The PHD ULTRA™ Setting the RS-232 Baud Rate For attaching the PHD Ultra to an external device using RS-232 serial communications, you can easily adjust the baud rate to optimize communications. TO ADJUST THE RS-232 COMMUNICATIONS BAUD RATE: 1. From the Quick Start or Method Main screen, choose the Settings button. 2. From the Settings menu, choose the RS-232 Baud Rate button to enter the Set Baud Rate screen. 3. Select the desired preset level value (9600, 19200, 38400, 57600, 115200).
Setting Up The PHD ULTRA™ Maintenance/Calibration Reminders The PHD Ultra monitors usage and provides convenient reminders for periodic maintenance or calibration. You can adjust the settings for the reminders to accommodate your specific operating conditions. Harvard Apparatus recommends that system calibration checks/adjustments be performed at least once per year on the pump and that lubrication should be performed once every 200 hours of operation.
Setting Up The PHD ULTRA™ Power Up Running The Power Up Running Setting is designed to allow the pump to restart pump operation automatically after a power outage when operating in a “continuous” mode. The Power Up Running selection is only valid under the following conditions: • When operating in a Quick Start Infuse Only or Withdraw Only method. • When the pump is running at the time of a power loss. TO SET POWER UP RUNNING: 1. From the Quick Start or Method Main screen, choose the Settings button.
Setting Up The PHD ULTRA™ SYRINGE LOADING 11 1 2 4 5 6 12 3 10 7 8 Figure 18: 1. 2. 3. 5. 6. 7. 8. Release Tabs Pusher Block 3. Syringe Holder Block 4. Retaining Bracket (Pusher Block) 5. Retaining Bracket (Syringe Holder Block) 6. Syringe Barrel Clamp Knob 7-8. Bracket Clamping Knobs 9. Mechanical Stop (Infuse) 10. Mechanical Stop (Withdraw) 11. Pulley Cover (Allen Wrench Holder) 12.
Setting Up The PHD ULTRA™ Mechanical Stop Mechanical Stop Figure 20. Syringe fully mounted and locked in place. Note mechanical stops set in position to prevent excess travel on pusher block. Changing the bracket for small (<30ml) vs large (>30ml) syringes: 1. Remove the Syringe Barrel Clamp Assembly by completely unscrewing the Syringe Barrel Clamp Knob. 2. Unscrew and remove the Retaining Nut and slide the clamp bar off the Syringe Barrel Clamp Knob. 3.
Using The PHD ULTRA™ Using The PHD ULTRA™ PUMP OPERATION OVERVIEW The following diagram provides an overview of basic operation for the PHD ULTRA™: S TEP 1 : Select A Method Choose an operating Method which defines the syringe being used, the direction (infuse or withdraw) and rate of flow, as well as any dynamic control of external devices. If one does not exist, you will create a new Method.
Using The PHD ULTRA™ • Multiple infusions • Ramped or stepped infusion rates • Periodic dispensing loops • Combination infuse and withdraw profiles • External system control or response to external signals METHOD TYPES EZ PRO Quick Start EZ PRO Quick Start Methods are designed for simple infusion or withdrawal applications, pumping at a constant flow rate for either a predetermined time period until a preset volume is pumped, or running in continuous operation until stopped.
Using The PHD ULTRA™ RUNNING THE PUMP Controlling The Pump The software provides Fast Forward (Infuse) and Fast Reverse (Withdraw) buttons that can be used to manually control the Pusher block and either Withdraw contents into the syringe or Infuse contents from the syringe. ✐ Use this button to advance the Pusher bar in the reverse (withdraw) direction. Use this button to advance the Pusher Bar in the forward (infuse) direction.
Using The PHD ULTRA™ Figure 23: Method Main screen showing a sample Method with 10 steps. SYRINGE SELECTION In order to accurately control the flow rate or volume delivery of the pump, the PHD ULTRA™ requires that each method include accurate setting of the syringe diameter and volume. All Methods (Quick Start, Preloaded, User-Defined) require a syringe definition, either by selecting from the installed syringe specifications list or by defining a new (Custom) syringe in the application.
Using The PHD ULTRA™ 2. From the Syringe Selection menu, double tap the Manufacturer/Model button for the desired syringe to enter the Syringe Size Selection screen. Figure 25: 3. Syringe Size Selection screen showing barrel dimensions and syringe capacity. Double tap the dimensions/capacity button for the syringe you will be using. TO CREATE A CUSTOM SYRINGE OPTION: 1. From the Quick Start or Method Main screen, choose the Syringe Select button. 2.
Using The PHD ULTRA™ Controlling The Pump Using A Stored Method The PHD ULTRA™ Methods contain the detailed parameters required to control the pump for infuse or withdraw operation. This section describes the basic operation of the pump using a stored Method. SELECTING A METHOD 1. From either the Quick Start or the Method Main screen, choose Method Select to access the Method Selection screen.
Using The PHD ULTRA™ PREVIEW THE METHOD 7. To Preview a Method, press the Method Preview button to enter the Run (Quick Start Methods) or Method Run (Preloaded and User-Defined Methods) screen. Figure 28: Quick Start Run screen for a Withdraw/Infuse Method. Figure 29: Method Run screen for Preloaded and User-Defined Methods.
Using The PHD ULTRA™ RUN THE METHOD 9. To start a pump Method, choose the Method Run button. While a Method is running, the Pump Run LED will be lit.
Using The PHD ULTRA™ Figure 32: User-Defined Method Run screen with Method paused on Step 1. Note the currently active Step is highlighted. 11. To Resume a Method that is paused, choose the Method Run button. 12. To Cancel a Method that is paused, choose the Method Clear button. The Method is reset to its initial parameters when Clear is pressed. 13. Upon completion of a Method operation, choose the Re-Run button to clear all counters and timers and repeat the Method.
Using The PHD ULTRA™ USING AN EZ PRO QUICK START METHOD Quick Start Methods are designed to help you quickly and conveniently use the PHD ULTRA™ for simple infusion or withdrawal applications. Each Quick Start Method requires a minimal number of user entries to operate.
Using The PHD ULTRA™ 2. Choose the Syringe Select button to enter the Syringe Selection screen. 3. Follow the instructions in the Syringe Selection section of this Guide to complete the selection of a syringe from the built-in syringe definition list or to create a new syringe definition. When finished, choose the Accept button to save your changes, or choose the Cancel button to cancel your changes, and return to the Quick Start screen. 4.
Using The PHD ULTRA™ Figure 36: Set Infuse and Withdraw Rates in a Quick Start Infuse and Withdraw combined Method. TO SET THE INFUSE RATE (INFUSE ONLY METHOD): 1. From the Quick Start or Method Main screen, choose the Infuse Rate Select button. 2. On the Set Infuse Rate screen, enter the desired rate value using the keypad or select the Minimum or Maximum Rate button. 3. If the Units of Rate are not correct for your application, choose the Units button. 4.
Using The PHD ULTRA™ TO SET THE TARGET VOLUME OR TIME: 1. From the Quick Start screen, choose the Target Volume/Time Select button. 2. On the Set Target Volume or Time screen, select the Volume or Time button, as desired. 3. Enter the desired value using the keypad, or select the Maximum Volume button. 4. If the Units of Volume/Time are not correct for your application, choose the Units button to the right of the parameter entry box.
Using The PHD ULTRA™ Preloaded/User-Defined Method Workflow The following flow diagram describes the use of an existing Preloaded or user-Defined Method: Select Method Select Syringe Model OR Create New Syringe Type Review Method Steps (Pump Operation Profiles) Enter Pump Operation Profile Operating Parameters Validate Commands and I/O Operations Figure 38: Preloaded/User-Defined Method workflow.
Using The PHD ULTRA™ STEP-BY-STEP OPERATION EXAMPLE: USING A PRELOADED OR USER-DEFINED METHOD The following steps describe operation using a Preloaded or User-Defined Method. In the following description, we are assuming the use of a Method that is already saved on the PHD ULTRA™. Note that if you make any changes to the Method’s operating parameters and choose Accept on the page where the changes are made, the changes automatically become part of the stored Method for future use. 1.
Using The PHD ULTRA™ Setting A Time Delay For Method Operation When using a Preloaded or User-Defined Method, you can set the PHD Ultra to run the Method at a specific date and time in the future. This delayed run function allows you to set up all the parameters for operation, then have the pump start unattended at the required time. Figure 40: Set Delay Date and Time screen. SETTING A TIME DELAY FOR METHOD OPERATION: 1.
Using The PHD ULTRA™ CREATING AN EZ PRO NEW METHOD If none of the Methods stored on the PHD ULTRA™ match your requirements, you can create a new Method and store the Method for use.
Using The PHD ULTRA™ 4. When you have entered the desired name for your Method, choose the Accept button to return to the Method Main screen. Your new Method will now be listed in the Method Select button. 5. Choose the Syringe Select button and complete the description of the syringe by following the procedure outlined previously in the Syringe Selection section of this Guide. 6. To begin creating Pump Control Profile Steps, choose the Step Definition button to enter the Method Steps Summary screen.
Using The PHD ULTRA™ Modifying a Preloaded/User-Defined Method Once you have created a Method or have reviewed a Preloaded Method, you may have a need to modify the structure of the Method (i.e. the types of Steps and Commands included, not just changes in the operating parameters for a specific Step. The following procedures will help you to efficiently modify a stored Method. CHANGING THE ORDER OF STEPS IN A METHOD 1. 2. 3.
Using The PHD ULTRA™ DEFINING METHOD STEP PARAMETERS This section of the User Guide describes each of the available functions available for use in PHD ULTRA™ Preloaded and User-Defined Methods. The information in this section is designed to help you set the individual parameter values in each of the Method Step options and to properly use the Advanced Options commands.
Using The PHD ULTRA™ Pump Operation Profiles The PHD ULTRA™ Preloaded Methods and all User-Defined Methods use Pump Operation Profiles as part of the Methods controlling the pump. These profiles are combined with a syringe selection and any Commands or Input/Output triggers to create a complete Method. The following sections describe each profile along with instructions for setting up the profile.
Using The PHD ULTRA™ CONSTANT RATE PROFILE The Constant Rate profile is used to run the pump at a defined flow rate and for a specific pumping mode (infuse or withdraw) up to a target volume or for a defined time period. Flow Rate STOP Target Time or Total Volume Point Time | Volume Figure 46: Constant Rate Pump Operation Profile.
Using The PHD ULTRA™ 6. When all parameters are correctly set on the Set Rate screen, choose Accept to confirm your settings and return to Step Definition screen. 7. Choose the Set Target Volume or Time button to enter the Set Volume or Time screen. 8. On the Set Volume or Time screen, select the Volume or Time button, as desired. 9. Enter the desired value using the keypad, or select the Maximum Volume button. 10.
Using The PHD ULTRA™ RAMP PR OFILE The Ramp Rate profile is used to create a linearly increasing or decreasing flow rate for a single pumping mode (infuse or withdraw) over the course of a defined time period. Target Time Flow Rate STOP Time Figure 48: Ramp Rate Pump Operation Profile.
Using The PHD ULTRA™ Figure 50: Set Start and End Rates screen. 3. Select the Start Rate button (it should be selected by default as indicated by the active data entry box to the right of the button). Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button. 4. If the Units of Rate are not correct for your application, choose the Units button. 5.
Using The PHD ULTRA™ STEPPED PROFILE The Stepped Rate profile is used to create an increasing or decreasing flow rate that varies in a series of equal steps between a starting and ending flow rate. Rate # Steps Time | Volume Figure 51: Stepped Rate Pump Operation Profile.
Using The PHD ULTRA™ Figure 52:. Set Start and End Rate screen. 3. Select the Start Rate button (it should be selected by default as indicated by the active data entry box to the right of the button). Enter the flow rate value desired using the keypad or select the Minimum Rate or Maximum Rate button. 4. If the Units of Rate are not correct for your application, choose the Units button. 5.
Using The PHD ULTRA™ PULSE PR OFILE The Pulse Rate profile is used to create an oscillating infusion or withdrawal profile that varies between a starting flow rate and a second rate that can be either or higher or lower than the start rate.The flow rate is constant within each segment of the pulsed flow profile and the user defines the total number of repetitions (pulses) for the oscillating pattern.
Using The PHD ULTRA™ TO SET UP/EDIT A PULSE RATE PROFILE: 1. From the Step Definition screen for a Pulse Rate profile, choose the Set Mode button to toggle the Flow Mode between Infuse and Withdraw operation. 2. Choose the Set Rates button to enter the Set Pulse Rates screen. 3. Select the Rate 1 button (it should be selected by default as indicated by the active data entry box to the right of the button).
Using The PHD ULTRA™ BOLUS PROFILE The Bolus Rate profile is used to infuse a defined dose of fluid controlled either by total volume or by the total time the pump operates. Bolus profiles are used for infusion only. In a bolus profile, you set either the time the pump operates or the total volume to be delivered. The pump automatically defaults to the maximum pump speed based on the syringe selected, but it can be modified when defining the Step parameters.
Using The PHD ULTRA™ 4. On the Units Selection screen, select the unit of measure (ml, µl, nl or pl) and the unit of time (sec, min, hr) as appropriate, then choose Accept to confirm your selections. 5. Select either the Volume or Time button to determine how you will control the Bolus infusion. If you selected Time, continue with Step 6. If you selected Volume, proceed to Step 9. 6. On the Set Target Times screen, enter the desired value using the keypad. 7.
Using The PHD ULTRA™ CONCENTRATION PROFILE The Concentration flow profile is used to manage infusion of a defined concentration of drug or other liquid to a set dosage level based on the subject’s weight. Animal Weight Drug Concentration # Doses Time Between Doses Flow Rate Dose | Time Figure 58: Concentration Flow Pump Operation Profile.
Using The PHD ULTRA™ TO SET UP/EDIT A CONCENTRATION FLOW PROFILE: 1. From the Step Definition screen for a Concentration profile, choose the Animal Weight button to enter the Set Animal Weight screen. Figure 60: Set Animal weight screen in Concentration profile. 2. Enter the Weight in grams then choose the Accept button to return to the Step Definition screen. 3. Choose the Set Rate and Concentration button to enter the Set Rate and Concentration screen. Figure 61.
Using The PHD ULTRA™ 13. When all parameters on the Set Dose or Duration screen are correct, choose the Accept button to return to the Step Definition screen. 14. Choose the Set # Doses & Time Between Doses button to enter the Set # Doses & Time Lag screen. Figure 62: Set # Doses and Time Lag screen in Concentration profile. 15. Select the Doses button (it should be selected by default as indicated by the active data entry box to the right of the button).
Using The PHD ULTRA™ AU TOFILL PROFILE The Autofill flow profile is an automated infuse/withdraw or withdraw/infuse step that operates at constant, but separately defined, flow rates for the withdraw and infuse operations. The Autofill profile is designed to work in conjunction with an autofill valve that controls the switching of flows from a reservoir for refilling the syringe, to the target infusion subject.
Using The PHD ULTRA™ TO SET UP/EDIT AN AUTOFILL FLOW PROFILE: 1. From the Step Definition screen for an Autofill profile, choose the Set mode button to toggle between the Withdraw/Infuse (WD/INF) and Infuse/Withdraw (INF/WD) modes. Withdraw/Infuse begins by withdrawing into the syringe, following by infusing into the target. 2. Choose the Set Rates button to enter the Set Infuse/Withdraw Rate screen. 3.
Using The PHD ULTRA™ Incorporating Advanced Options (Commands and I/O) Into A Method PHD ULTRA™ Methods can incorporate a variety of commands that expand the versatility of the system by permitting convenient looping, chaining of events, sending signals to external devices, or accepting inbound signals from external devices. These Advanced Options controls are described in this section of the Guide. To access the Advanced Options, choose Advanced Options from the Select Profile screen.
Using The PHD ULTRA™ GO TO STEP The Go To Command is used to instruct the PHD ULTRA™ to jump to a particular Step in a Method. This can be used to help you loop back or jump forward in a Method protocol and can be used in conjunction with I/O Events to make the pump respond to a trigger from an external device. When defining a Go To jump in a Method, the Command targets the Step sequence position and not the function that currently occupies that position.
Using The PHD ULTRA™ DELAY The Delay Command is used to insert a time delay in a procedure. You can program a time delay between 0.2 seconds up to 99:99:99 (99 hours, 99 minutes, 99 seconds). TO INSERT A TIME DELAY IN A METHOD: 1. From the Method Main screen, choose Step Definition to enter the Method Steps Summary screen. 2. Highlight (select once) the Method Step that you want the time delay to follow. 3. Choose the Add Step button to access the Method Steps Menu screen. 4.
Using The PHD ULTRA™ REPEAT STEP The Repeat Command is used to repeat Steps in a procedure a defined number of times. To use this Command, you will tell the application what Steps you want to Repeat and how many times you want them repeated. The Repeat Step command creates a “jump” back to a specific Step in a Method that executes the defined Step plus all following Steps up to the Repeat command (i.e. it creates a Method loop). TO INSERT A STEP REPEAT SEQUENCE IN A METHOD: 1.
Using The PHD ULTRA™ LIN K METHOD The Link Command is used to insert a link to another stored Method into the operating sequence of a Method. You can Link a Method anyplace in the Method sequence. Figure 69: Advanced Options Link Command selection screen. TO LINK TO A STORED METHOD: 1. From the Method Main screen, choose Step Definition to enter the Method Steps Summary screen. 2. Highlight (select once) the Method Step that you want the Link Command to follow. 3.
Using The PHD ULTRA™ I/O EVENT TRIGGERS The PHD Ultra pump permits easy connection to external I/O devices via a variety of interfaces, including a built-in 15-pin D-sub connector. The PHD Ultra software incorporates simple commands for incorporating I/O controls into your pump Methods. Integrated commands for control of an external valve, multi-pump synchronization, and general trigger signals are integrated in the software. The following procedure describes the use of the Advanced Options I/O commands.
Using The PHD ULTRA™ TO INSERT AN I/O TRIGGER (OUT) COMMAND: 1. From the Method Main screen, choose Step Definition to enter the Method Steps Summary screen. 2. Highlight (select once) the Method Step that you want the I/O Trigger (Out) to follow. 3. Choose the Add Step button to access the Method Steps Menu screen. 4. Choose Advanced Options to enter the Select Advanced Options screen. 5. Choose the I/O Trigger (out) button to enter the Trigger Step Definition Screen. 6.
Using The PHD ULTRA™ I/O EVENT (IN) The I/O Event (in) command is used to have the pump pause and wait for a signal from an external device or for a manual user input at the touchscreen. These external signals are communicated via Pin 2 on the 15-pin I/O Connector port. Figure 71: I/O Event (in) Advanced Options menu. The following options are available when setting an I/O Event (in) command. • • • Input High-to-Low: Sets the pump to wait for a logic low input signal falling edge.
Using The PHD ULTRA™ I/O ON EVENT (IN) The I/O On Event (in) command is used to have the pump jump to a linked method if the signal is received.These events are communicated via Pin 2 on the 15-pin I/O Connector. Figure 72: I/O On Event (in) Advanced Options menu. The following options are available when setting an I/O On Event (in) command.
Using The PHD ULTRA™ MANAGING EZ PRO METHODS All Preloaded and User-Defined Methods are stored in the Method List for ongoing use. Over time, you may want to manage previously created Methods to keep your Method List easy to navigate. In addition, the PHD ULTRA™ allows you to export Method information to an externally connected pump or to a computer that will be used to control operation. This section of the Guide describes the file management facilities available on the PHD ULTRA™.
Using The PHD ULTRA™ Appending A Method Follow these instructions to Append a previously created Method into a new Method: 1. From the Quick Start or Method Main screen, choose the Method Select button to enter the Method Selection screen. 2. From the list of available Methods, select (press once) the Method you want as the base Method. 3. Choose the File Options button to enter the File Options screen. 4. Choose Append to access the Method to Append screen. 5.
Using The PHD ULTRA™ PHD Ultra Method Export and Import The PHD Ultra pump permits the chaining of multiple pumps, as well as integration with/control by an external computer through HyperTerminal. To facilitiate operation in these modes, the PHD Ultra application software includes a variety of commands designed to simplify the export/import of Methods between the pump and external devices. This section outlines the use of these commands.
Using The PHD ULTRA™ EXPORTING A METHOD TO AN EXTERNAL COMPUTER Follow these instructions to export a Method to an external computer. 1. Connect the pump to the external computer via a RS-232 or USB cable. 2. On the computer, open the HyperTerminal application and verify that the computer can communicate with the pump. 3. At the PHD Ultra, from the Method Main screen, choose Method Select then choose the Method you would like to export. 4. Choose the File Options button. 5.
Using The PHD ULTRA™ EXTERNAL PUMP CONTROL This section of the PHD Ultra manual describes the control of the pump using an external computer device. Setting Up HyperTerminal HyperTerminal is a Windows application designed to support the external control of devices such as the PHD Ultra pump through an RS-232 or USB connection. The following instructions describe the configuration of the HyperTerminal application. 1. Select “Start – All Programs – Accessories – Communications – HyperTerminal”. 2.
Using The PHD ULTRA™ 5. Verify the Settings are as shown above. 6. Choose ASCII Setup and select “Echo typed characters locally”then click OK to complete the setup.
Using The PHD ULTRA™ PUMP CHAIN COMMANDS The Pump Chain commands allow all pump control information to be managed from an external computer source. These commands can also be used to control a series of pumps (up to 100) from a single computer interface. PHD Ultra commands are communicated to the pump via the RS-232 or USB port interfaces through a terminal program such as HyperTerminal.
Using The PHD ULTRA™ dim Sets or displays the backlight level in percent. Valid range is 0 to 100. Note that specifying 0 will turn the backlight off so that the screen will not be visible. dim [0-100] echo Sets or displays the RS-232/USB echo state. Valid states are on or off. echo [on|off] free Displays the number of free method steps left on the disk. free force Sets or displays the infusion force level in percent. Valid range is 1 to 100. force [1-100] poll Sets or displays the polling mode state.
Using The PHD ULTRA™ Run Commands irun Runs the pump in the infuse direction. irun wrun Runs the pump in the withdraw direction. wrun rrun Runs the pump in the opposite direction. rrun run Simulates a key press of the run button located in the lower right corner of the screen. run stop / stp Stops the pump. stop stp Rate Commands crate Displays the current rate that the motor is running at. If the motor is stopped, the current rate is 0. crate diameter Sets or displays the syringe diameter in mm.
Using The PHD ULTRA™ Volume Commands civolume Clears the infused volume. civolume ctvolume Clears the target volume. ctvolume cvolume Clears both the infused and withdrawn volumes. cvolume cwvolume Clears the withdrawn volume. cwvolume ivolume Displays the infused volume. ivolume tvolume Sets or displays the target volume. tvolume [{target volume} {volume units}] wvolume Displays the withdrawn volume.
Using The PHD ULTRA™ Time Commands citime Clears the infused time. citime ctime Clears both the infused and withdrawn times. ctime cttime Clears the target time. cttime cwtime Clears the withdrawn time. cwtime itime Displays the infused time. itime ttime Sets or displays the target time. ttime [{target time} {time units}] wtime Displays the withdrawn time. wtime Digital I/O Commands input Reads and displays the trigger input port status. input output Sets the level on one of the output ports.
Using The PHD ULTRA™ Internal Commands Internal commands are used by the pump but are not meant to be entered by the user. They are listed here as additional information. method Defines the file header for an exported method. method {hex data} mend Defines the end of an exported method transfer. mend mstep Defines a step in an exported method. mstep {hex data} status Displays the raw status for a controlling computer.
Appendices Appendices APPENDIX A: SYRINGE VOLUME/DIAMETER REFERENCE TABLE Harvard Apparatus Stainless Steel Size 2.5 ml 8 20 50 100 Diameter 4.851 mm 9.525 19.13 28.6 34.9 Air–Tite HSW Norm-Ject Size 1. ml 2.5 5 10 20 30 50 Diameter 4.69 mm 9.65 12.45 15.9 20.05 22.9 29.2 Becton Dickinson ‘Plasti-pak’ Size 1. ml 3 5 10 20 30 50 60 Size 1. ml 3 5 10 20 30 60 Diameter 4.699 mm 8.585 11.989 14.427 19.05 21.59 26.594 26.594 Terumo Diameter 4.70 mm 8.95 mm 13 15.8 20.15 23.1 29.
Appendices APPENDIX B: STAINLESS STEEL SYRINGES Harvard High Pressure Stainless Steel Syringes Harvard offers a complete line (20 to 200ml; for 2.5 & 8ml see below) of Stainless Steel Syringes intended for high pressure applications with good resistance to most aggressive liquids. Wetted parts are #316 stainless steel or Viton. Syringes are available in 20, 50, 100, and 200 ml sizes with removable replacement tips. Syringe to tube fittings are available in 1/16, 1/8, and 1/4 inch sizes.
Appendices APPENDIX C: MIN/MAX FLOW RATES Nominal Minimum/Maximum Flow Rates for Various Syringes. (Actual Limits will vary depending on syringe manufacturer) Syringe Size Syringe ID* Minimum Rate 1 µl 0.146 mm 3.000 0.5 µl 2 µl 5 µl 10 µl 25 µl 50 µl 0.103 mm 0.206 mm 1 ml 3 ml 33.300 1.03 mm 150.300 2.304 mm 752.100 3.256 mm 1000 µl pl/min 75.240 500 µl 250 µl 4.608 mm 4.699 mm 300.720 1.502 3.008 3.128 8.585 mm 10.442 10 ml 14.427 mm 29.490 30 ml 21.59 5 ml 20 ml 11.
Appendices APPENDIX D: SYRINGE HOLDER OPTIONS Mounting a 4x140 Multi-Rack Figure 74: 4x140 Multi-Rack Syringe Holder Assembly. 1. Remove Syringe Clamp bracket from the Syringe Holder by unscrewing knob. 2. Attach the Multi-Retainer to the Multi-Holder using T-Knobs (2) with Spring Washers (2). 3. Place the Multi-Holder on the Dual Syringe Holder and attach using #10-32 x ½” Screws (4). 4. Attach Clamp Bar by screwing the Multi-Syringe Clamp Knob into the threaded bar attached to the Multi-Holder.
Appendices Figure 75: 4x140 Multi-Rack Pusher Block Assembly. 1. Remove Dual Syringe Anti-Syphon Bracket by unscrewing the Bracket Clamping Knob completely and removing the Bracket Clamping Shaft. 2. Attach the Pusher Bar to the Pusher Block using flat head screws (2). 3. Attach the Anti-Syphon Bracket to the pusher bar using the Thumbscrews (3), Washers (3), and Springs (3). Slide Thumbscrews through the Pusher Bar holes, slide Washer then Spring over Thumbscrews and tighten to the Pusher Bar.
Appendices Mounting a 6x10 Multi-Rack Figure 76: 1. 6x10 Multi-Rack Syringe Holder Assembly. Remove Syringe Clamp bracket from the Syringe Holder by unscrewing knob. 2. Attach the Multi-Retainer to the Multi-Holder using T-Knobs (2) with Spring Washers (2). 3. Place the Multi-Holder on the Dual Syringe Holder and attach using #10-32 x ½” Screws (4). 4. Attach Clamp Bar by screwing the Multi-Syringe Clamp Knob into the threaded bar attached to the Multi-Holder.
Appendices Figure 77: 6x10 Multi-Rack Pusher Block Assembly. 1. Remove Dual Syringe Anti-Syphon Bracket by unscrewing the Bracket Clamping Knob completely and removing the Bracket Clamping Shaft. 2. Attach the Pusher Bar to the Pusher Block using flat head screws (2). 3. Assemble the Hold-Down Bracket to the 6x10 Anti-Syphon Bracket using Thumbscrews (2). 4. Attach the Anti-Syphon Bracket Assembly to the pusher bar using the Thumbscrews (3), Washers (3), and Springs (3).
Appendices Syringe Loading – 4 x 140 & 6 x 10 Multi-Racks 6 11 5 4 7 2 3 1 12 8 10 9 ✐ Figure 78: 1. Release Tabs 2. Pusher Block 3. Syringe Holder 4. Retaining Bracket (Syringe Holder) 5. Anti-Syphon Bar 6. Pusher Bar 7. Clamp Bar 8. T-Knobs 9. Mechanical Stop (Infuse) 10. Mechanical Stop (Withdraw) 11. Plunger Holder 12. Pulley Cover (Allen Wrench Holder) Front view of PHD ULTRA™ showing controls for multi-rack syringe loading.
Appendices 11. Squeeze the Release Tabs (1) and move the Pusher Block (2) to the left until the plunger is located just to the left of the maximum volume allowable. Use the Allen Wrench to loosen the refill Mechanical Stop (10) and slide it to the right until it is contact with the Pusher Block (2). Tighten the Withdraw Mechanical Stop (10) and then return the Allen Wrench to its holder (12). ✐ Figure 79: 4 x 140 Multi-Rack 6 x 10 Multi-Rack Multi-Rack Configurations.
Appendices Mounting a Micro Liter Multi-Rack Figure 80: 1. Micro Liter Multi-Rack Syringe Holder Assembly. Remove Syringe Clamp and Knob from the Syringe Holder. 2. First assemble the Retaining Bracket to the Micro Holder using the two T-Knobs. 3. Place the assembled Micro Holder onto the syringe holder and screw in the two Standoff Bars. 4. Place the Clamp Bar Assembly onto the Micro Holder by screwing the Thumbscrew Caps into the Standoff Bars.
Appendices Figure 81: Micro Liter Multi-Rack Pusher Block Assembly. 5. Remove the Pusher Blocks Retaining Bracket by unscrewing the Bracket Clamping Knob completely and removing the Clamp Shaft. 6. Attach Anti-Syphon Bracket using the Clamp Shaft and Bracket Clamping Knob.
Appendices Syringe Loading – Micro Liter Multi-Rack 1 2 5 3 4 1 7 8 1 9 1. Release Tabs 4. Bracket Clamping Knob 8. Syringe Holder 2. Pusher Block 5. Thumbscrew Cap 9. T-Knob 3. Retaining Bracket (Pusher Block) 6. Screw Cap 10. Bumper 7. Retaining Bracket (Syringe Holder) 11. Clamp Bar Assembly (Allen Wrench Holder) Figure 82: Front view of PHD ULTRA™ showing controls for micro liter multi-rack syringe loading. 1.
Appendices Figure 83: Micro Liter Multi-Rack Configuration. Syringe Loading – Push-Pull Mechanism 8 4 6 5 7 1. 1 3 2 4 Figure 84: Thumb Nut (Syringe Holder) 2. Adjustable Syringe Holder 3. Syringe Clamp 4. Bracket Clamping Knobs (Syringe Holder) 5. Bracket Clamping Knob (Pusher Block) 6. Retaining Bracket (Syringe Holder) 7. Retaining Bracket (Pusher Block) 8. Thumb Nut (Syringe Clamp) PHD Ultra showing Push-Pull Mechanism installed. To load syringes on the push/pull end: 1.
Appendices ✐ Figure 85: PHD Ultra showing syringes installed on both sides of the Pusher Block. NOTE: For Syringes <10ml you will need to remove the Thumb Nut (8) and then remove and flip over the Syringe Clamp (3). Remote Unit Connection Connect the control unit to the remote pump unit with the cable provided. Figure 3: Remote unit connection.
Appendices APPENDIX E: EXTERNAL CONNECTIONS User I/O Connector Specifications Pin Assignments 1. Direction control input Rising edge sets pump to infuse Falling edge sets pump to refill 2. Trigger Input Event trigger – falling edge triggers a program event 3. Footswitch Input Momentary switch closure to ground or TTL Logic Low; falling edge toggles between states (run/stop) 4. Trigger 1 Output TTL Logic Output-Default = Low Control thru method or serial comm 5.
Appendices Analog Input Pin # Signal Description 2 IN - (SIG RTN) Signal RTN 1 4 3 2 1 N/C 4 IN + (ANALOG IN) ANALOG IN (SIG + ) OVERVOLTAGE RATING (Absolute Max/Min): 10V ! Figure 87: 3 GND Ground 0-10 V Analog In 0 to +10V +15V Max. -2.5V Min. 4-Pin miniDIN Female Analog control of the motor speed can be achieved in many ways. The following circuit is recommended. A DC voltage (10 VDC max) will be needed to supply the excitation voltage to the circuit.
Appendices USB Mating Face Figure 89: 2 1 3 4 Pin # Signal 1 +5V 3 + Data 2 4 - Data GND USB Type B Female USB VIRTUAL COMMPORT DRIVER INSTALLATION When you connect the PHD Ultra pump to a computer via USB, Windows will seek to install a driver for communication.The following section details the installation of the Virtual CommPort Driver supplied with the PHD Ultra pump. 1.
Appendices 2. Choose “Install from a list or specific location (Advanced)” and click Next. 3. Browse for the proper driver installation file and then click next. It can be found on the CD supplied with the PHD ULTRA™ Pump. Windows XP or Vista: PHDUltra-XPVista.inf Windows 2000: PHDUltra-w2k.inf. 4. If a warning message regarding Windows Logo testing is displayed, choose Continue Anyway to proceed with the installation. 5. The Virtual Commport software will be installed.
Appendices RS-232 Specifications Pin # 2 1 6 Figure 90: 4 3 7 8 2 TXD 5 GND 3 5 9 Signal 1, 4, 6-9 RXD N/C RS-232 9-Pin D-Sub Male Baud Rates: 9600 19200 38400 57600 115200 Word size – 8 Parity – None Stop Bits – 2 Pump Figure 91: PC RS-232 Specifications.
Appendices Legacy RJ-11 Option Transmit Ground Ground Receive Receive Transmit Pump Port #2 (out) Figure 92: P.C. Pumps with Legacy RJ-11 Option. RS-232 D-Sub 9 F/F Cable Cat # 70-4004 RS-232 IN PUMP D-Sub 9 ADDR Ø RJ-11 OUT OR P.C.
Appendices RS-485 CAUTION: DO NOT CONNECT TO FIREWIRE PORTS ON A PC. DAMAGE MAY OCCUR TO PUMP AND/OR PC. ! Mating Face Pin # 1 2 1 2 3 4 3 6 5 5 Figure 94: P.C.
Appendices APPENDIX F: MAINTENANCE Maintenance PHD ULTRA™ series pumps require no special maintenance other than keeping them clean by avoiding accidental spills of pumped material. Refer to the chemical compatibility chart below for specific chemical resistance information. The two guide rods and the lead screw should be sparingly lubricated periodically; every 200 hrs the pump will remind you.The guide rods and the lead screw should be lubricated with Super Lube Synthetic Grease provided with the pump.
Appendices Fuse Replacement Key Information 1. Make sure the power cord is disconnected from the main supply before servicing the fuse. 2. Use only Type 3AG, 1/4" x 11/4, ½ amp, 250 volt;Type T (time delay) fuses (Harvard Apparatus Part No. 5146-037 or equivalent). Turn off power and remove power cord from power module. Use a straight blade screwdriver to pry open the access door. Remove the fuse holder and then remove the fuses from this holder as shown in figure below.
Appendices Upgrading EZ PRO Software 1. Upload the latest software version to your desktop (format is filename.dfu). 2. Connect the pump to PC using a USB cable. Open HyperTerminal and verify communication with the pump. 3. ✐ Type “boot” into HyperTerminal.The pump’s screen will be blank and the light should be flashing. NOTE: First time users will need to install the PHD ULTRA™ USB driver as well as the bootloader driver file (PHDUltra-Bootloader.inf).
Appendices Troubleshooting Infusion Accuracy To ensure infusion accuracy always use new syringe(s) and measure syringe bore diameter and enter actual dimensions in millimeters (mm) using the Custom Syringe entry option. Additionally, make sure that the guide rods and lead screw are properly lubricated. RS-232 Difficulties Verify that the baud rates, data framing parameters, data bits, parity, and stop bits on all devices are the same. Verify flow control (handshaking) is set to None.
Appendices PHD ULTRA™ Display Error Messages Motor Stalled This indicates that the pusher block travel has been impeded. This may be caused by the syringe plunger hitting bottom, a kink in the tubing (occlusion), syringe plunger binding or any situation requiring more force to the head of the syringe than the pump is capable of delivering. To restart the pump, remove the cause of stalling and/or increase the force setting, then press RUN. The pump will continue the interrupted procedure where it stopped.
Appendices APPENDIX G: ‘AUTO FILL’ VALVES There are two 'Auto Fill' Valve models available: Catalog No. Product 55-7002 Supplied with 1/8 inch ID, 1/4 inch OD silastic tubing and connector to syringe 70-4005 Adapter, PHD Digital I/O 55-7004 High Pressure: Supplied with 1/4 inch OD 304 stainless steel tubing with Swagelok fittings Setting Up the Valve Set up consists of three phases: Voltage selection (110 or 220V), Pump to valve electrical connection and Valve tubing to syringe hook up. 1. 2. 3.
Appendices APPENDIX H: ORDERING INFORMATION Standard Version Description Stand Alone PHD ULTRA™ Infuse Only 70-3005 PHD ULTRA Infuse/Withdraw ™ PHD ULTRA™ Infuse/Withdraw Programmable Remote 70-3105 70-3006 70-3106 Stand Alone Remote 70-3007 70-3107 Push/Pull Versions Description PHD ULTRA™ w/ Push/Pull Mechanism PHD ULTRA Programmable w/ Push/Pull Mechanism ™ 70-3008 70-3009 70-3108 70-3109 Rack Options Catalog # Description 70-3021 4x140 Multi Rack 70-3020 70-3022 70-3023 6x10
Appendices Accessories Catalog # 70-4000 Description 70-4001 70-4002 RS-485 Cable for Pump-to-Pump Communication, 2m USB Cable for PC-to-Pump Communication, 2 m 70-4003 RS-485 Cable for Pump-to-Pump Communication, 0.5m USB Cable for PC-to-Pump Communication, 5m 70-4004 RS-232 Cable for PC-to-Pump Communication, 9 pin D-sub, 2m 72-1405 Remote Extension Cable, 9.1 m (30 ft) 72-0199 Remote Extension Cable, 1.
Declaration of Conformity Manufacturer: Harvard Apparatus, Inc. 84 October Hill Road Holliston, Massachusetts 01746-1388, U.S.A. Phone: (508) 893-8999 We herewith declare that the following product: Product Name: Model No.
Harvard Apparatus pumps are distributed on a non-exclusive basis by: Instech Laboratories, Inc. Headquarters: 5209 Militia Hill Road Plymouth Meeting, PA 19462 TL (800) 443-4227 TL (610) 941-0132 FX (610) 941-0134 European sales office: P.O. Box 2 9950 AA Winsum The Netherlands TL +31 (0)594 769026 www.instechlabs.
