Technical data

Agilent 1290 Infinity LC System Manual and Quick Reference 99
Quick Start Guide
5
Data Acquisition in Method and Run Control View
b Other parameters can remain at default settings. Click OK to exit the
window.
The changes are sent to the autosampler module.
4 Right-click the Thermostatted Column Compartment (TCC) area, and select
Method... in the context menu.
a In the Method page for the 1290 Infinity TCC, enter the following
parameters:
• Left Temperature 40 °C
• Right Temperature Combined
b Other parameters can remain at default settings. Click OK to exit the
window.
The changes are sent to the TCC module.
5 Right-click the Diode-Array Detector area, and select Method... in the
context menu.
a In the Method page for the 1290 Infinity DAD , enter the following
parameters:
• Use Signal: Turn all signals except Signal A off by clearing the check
boxes.
• Signal A: 250 nm, bw 100 nm, ref 360 nm, bw 100 nm
• Peak width: 0.012 min (0.25 s Response, 20 Hz)
b In the Advanced section, set Spectrum Store to All.
c Other parameters can remain at default settings. Click OK to exit the
window.
The changes are sent to the DAD module.
6 All the required module parameters have now been entered. Select Method >
Save Method As... to save the method with a new name.
The ChemStation will not allow the method to be saved as DEF_LC.M, so that
the default method template is not altered.
7 Allow the system to equilibrate for at least 10 min, and check that the
baseline in the Online Plot is stable before starting the analysis.