Technical data

Agilent 1290 Infinity LC System Manual and Quick Reference 97
Quick Start Guide
5
Data Acquisition in Method and Run Control View
Tabl e 9 Method parameters for first separation run
Module Parameter Setting
Pump Solvent A Water
Solvent B Acetonitrile
Flow rate 0.4 ml/min
Initial Composition 60 % A, 40 % B
Gradient Timetable At 4 minutes 20 % A, 80 % B
Stop Time 5 minutes
Autosampler Injection 1 µl
Needle wash Flush port, 6 s
TCC Column ZORBAX Eclipse Plus C18
1.8 µm, 2.1 mm x 50 mm i.d.
Te m p e r a t u r e 4 0 ° C
Detector Signal A 250 nm, bw 100 nm, ref
360 nm, bw 100 nm
Peak width 0.025 min (10 Hz)
Store Spectra All