Technical data

Agilent 1290 Infinity LC System Manual and Quick Reference 69
Optimization of the Agilent 1290 Infinity LC System
3
How to Prevent Column Blockages
9 Purge the pumps (the connections up to the column) of any buffer
containing mobile phases and flush through 5 ml of solvent before attaching
the column to the instrument.
10 Flush the column with compatible mobile phase starting slowly at
0.1 ml/min for a 2.1 mm inner diameter column, 0.2 ml/min for a 3.0 mm
inner diameter column, and 0.4 ml/min for 4.6 mm inner diameter. Increase
the flow rate to the desired flow over 5 minutes.
11 Once the pressure has stabilized, attach the column to the detector.
12 Equilibrate the column and detector with 10 column volumes of the mobile
phase prior to use (see Table 7 on page 44 for column volumes).
13 Avoid over-pressure. Check the pressure range of your gradient before
starting any sequence.
14 If samples are dissolved in high organic content consider using an injection
program to add a plug of weaker solvent before and after the sample aliquot
to reduce the risk of precipitation in high concentration buffer mobile
phase.