Technical data

Agilent 1290 Infinity LC System Manual and Quick Reference 43
Optimization of the Agilent 1290 Infinity LC System
3
How to Configure the Optimum Delay Volume
Tabl e 4 Delay volumes of 1290 Infinity Binary LC system configurations
System Configurations
1
1
added 5 µl to allow for connections in system configurations
Delay Volume (µl)
Binary Pump + Fixed Loop Autosampler (MS only) 20
Binary Pump + Jet Weaver + Fixed Loop 55
Binary Pump + standard Autosampler (MS only) 90
Binary Pump + Jet Weaver + Autosampler 125
Tabl e 5 Delay volumes of 1290 Infinity Quaternary LC system configurations
System Configurations
1
1
added 5 µl to allow for connections in system configurations
Delay Volume (µl)
Quaternary Pump + Fixed Loop Autosampler (MS only) 360
Quaternary Pump + standard Autosampler (MS only) 430
Quaternary Pump + V380 Jet Weaver + Fixed Loop 510
Quaternary Pump + V380 Jet Weaver + Autosampler 580
Tabl e 6 System delay times for gradient to reach head of column
Flow
Rate
(ml/min)
System Delay Volume (microliters)
20 55 90 125 360 395 430 465
Delay Times (minutes)
0.2 0.10 0.28 0.43 0.60 1.80 2.15 2.55 2.90
0.4 0.05 0.14 0.21 0.30 0.90 1.08 1.28 1.45
0.6 0.03 0.09 0.14 0.20 0.60 0.72 0.85 0.97
0.8 0.03 0.07 0.11 0.15 0.45 0.54 0.64 0.73
1.0 0.02 0.06 0.09 0.12 0.36 0.43 0.51 0.58