Technical data

42 Agilent 1290 Infinity LC System Manual and Quick Reference

3 Optimization of the Agilent 1290 Infinity LC System

How to Configure the Optimum Delay Volume

Table 3 on page 42 and Table 4 on page 43 show the component volumes which

contribute to system delay volume in the Agilent 1290 Infinity LC System. In

the standard configuration with the Agilent 1290 Infinity Binary Pump, with

the Jet Weaver mixer, the 1290 Infinity Autosampler and Thermostatted

Column Compartment the system delay volume is about 125 µl. This standard

delay volume is suitable for the majority of applications. For example, a fast

separation on a 50 mm x 2.1 mm column packed with sub-two-micron

particles at a moderate flow rate of 0.6 ml/min will result in a typical gradient

delay time of about 0.2 min which is generally acceptable with gradient times

of two to three minutes (see Table 6 on page 43). It is often useful to consider

the flow rate in terms of column volumes and it can be seen from Table 7 on

page 44 that with this column at 0.6 ml/min about 6 column volumes per

minute flow through the system and the delay volume is about 1.2 times the

column volume.

A setup with the Agilent 1290 Infinity Quaternary Pump, the 1290 Infinity

Autosampler, and Thermostatted Column Compartment has a delay volume of

430 µL, resulting in a delay time of 0.7 min. This is the limit for acceptance of

gradient times of 3 min.

Tabl e 3 Delay volumes of 1290 Infinity LC modules

Components Delay Volume (µl)

Binary Pump 10

Jet Weaver mixer (standard) 35

Binary Pump + Jet Weaver 45

Quaternary Pump 350

Quaternary Pump + V380 Jet Weaver 500

Autosampler (fixed 5 µl loop) 5

Autosampler (standard, variable volume) 80

Column Compartment low-dispersion heat exchanger 1.6

Connection tubing, 0.12 mm i.d., per 100 mm 1.1