Technical data

Agilent 1290 Infinity LC System Manual and Quick Reference 37

The Agilent 1290 Infinity LC System - Product Description

System Components

The module also incorporates electronic temperature control to further

enhance the resistance to ambient temperature effects. Although the hydraulic

volume of the Max-Light cartridge cell is very small, the path length is a

standard 10 mm. However, for even higher sensitivity the alternative Agilent

Max-Light high sensitivity cell is available with a path length of 60 mm. Cells

are easily exchanged by sliding them in or out of the cell holder and they are

auto-aligned in the optical bench. The DAD light source is a deuterium lamp

and the operating wavelength range covered is 190 to 640 nm. This is detected

by a diode-array comprising 1024 diodes. The entrance to the spectrograph is

through a programmable optical slit which can give spectral resolution from 1

to 8 nm. This is generally operated in the middle of the range but for

optimization can be closed down to 1nm for high spectral resolution (rarely

required in liquid phase UV spectra) or opened up to 8 nm for maximum light

transmission and minimum noise in the signal.

The chromatographic signals are extracted from the diode-array data within

the firmware of the module. Up to 8 individual signals can be defined, each

comprising a signal wavelength, a diode-bunching bandwidth and, if required,

a reference wavelength and bandwidth. Signals can be output at up to 160 Hz

(160 data points/second) for accurate recording of the fastest (narrowest)

chromatographic peaks. At the same time the module can also output

full-range spectra to the data system at the same rate of 160 Hz.

For regulated laboratories it is important that all the method parameters are

recorded. The 1290 Infinity DAD not only records the instrument setpoints but

also has RFID tags (radio-frequency identification tags) incorporated into the

lamp and flow cell cartridge so that the identity and variables of these

important components are also recorded by the system.