Technical data

Agilent 1290 Infinity LC System Manual and Quick Reference 131
Appendix
6
Setting Up a Method using Edit Entire Method
The entrance slit to the spectrograph controls the spectral resolution and
influences the baseline noise and sensitivity. The default setting is 4 nm,
which is suitable for most applications. See “How to Achieve Higher
Sensitivity” on page 59for further discussion of this parameter.
• Advanced - Autobalance sets the absorbance level to zero at all wavelengths
(that is, balances all points on the spectrum to zero) and hence also zeroes
the baseline signal. Prerun is selected to balance just before the start of the
run, which is the normal situation. Sometimes Postrun is alternatively
selected to balance at the end of the run after the Postrun Time has expired.
For example, if the signal always shows a negative drift and the user prefers
the run to end at zero absorbance, then this will set the correct zero level
for the following run. It will not retrospectively change the run at the end of
which it performed the balance.
• Advanced - Lamps on required for analysis: the 1290 Infinity DAD or MWD has
one deuterium lamp and it needs to be on for analysis so this box should be
checked.