Technical data

Agilent 1290 Infinity LC System Manual and Quick Reference 13
Introduction to Ultra-High Performance Liquid Chromatography
1
Theory of Using Smaller Particles in Liquid Chromatography
Agilent already offered more than 140 ZORBAX 1.8 µm Rapid Resolution High
Throughput (RRHT) columns (14 selectivity choices; 15 to 150 mm long; 2.1,
3.0 and 4.6 mm internal diameters) and with the launch of the Agilent 1290
Infinity LC the STM range is extended to include the Rapid Resolution High
Definition (RRHD) 1200 bar columns. This enables the optimum stationary
phase to be selected so that the selectivity is maximized. The resolution, flow
rate and analysis time can be optimized by selecting the appropriate column
length and diameter and operation with longer STM columns has become more
accessible than ever before.
Many laboratories perform an extensive screening process to select the best
combination of stationary phase, mobile phase and temperature for their
separations. Agilent offers Method Development Solutions on both the 1200
Series and 1290 Infinity LC systems. These offer complete automation of this
time consuming selection process – making method development and method
transfer an easier and more reliable task.
ZORBAX 1.8 µm RRHD and RRHT columns use the same chemistry as
ZORBAX columns with 3.5 and 5 µm particles. As a result, for any particular
ZORBAX phase, the 5.0, 3.5 and 1.8 µm particles provide identical selectivity,
which allows easy, fast and secure bidirectional method transfer between
conventional LC, UHPLC and preparative LC.