Technical data

Agilent 1290 Infinity LC System Manual and Quick Reference 119
Appendix
6
Setting Up a Method using Edit Entire Method
Autosampler tab (HiP-ALS)
Figure 37 Setup Method screen –High Performance Autosampler tab
•Injection Mode
• Injection volume sets the volume to be injected (example 3 µl),
• Standard injection indicates that no external needle wash is done,
• Injection with needle wash is used to reduce potential carryover. This is the
recommended option and is configured in the next entry.
• Needle Wash – if selected above.
• Mode determines how the outside of the needle will be rinsed: either
actively in the Flush Port or by dipping into a specified Wash Vial.
• Time in seconds the peristaltic pump connected to the flush port will
pump the rinse solvent. It then pumps for a further 15 s to clear the flush
port.