Technical data

100 Agilent 1290 Infinity LC System Manual and Quick Reference
5 Quick Start Guide
Data Acquisition in Method and Run Control View
Running the Method for a Single Injection
This section shows how to run a single injection of the test mix using the
conditions entered in the previous section.
Analyses on the ChemStation can be run in two modes:
•Run Method — single injections, for example, in interactive method
development, using the parameter settings current at the time;
•Run Sequence — automated series of injections from multiple vials, possibly
with multiple methods. For further details refer to the ChemStation
manuals.
1 Click the Select Run Method Task icon .
2 If required method conditions are not currently loaded, select Method > Load
Method or the icon under the menu bar to load them.
3 Place the sample vial in position 1 — this is the front position of the 10 x
2 ml vial positions on the right hand side of the sample tray.
4 Select Run Control > Sample Info, and enter a Subdirectory name (optional),
Filename, Sample Location (
vial 1), Sample Name and any Comment.
5 If the system is already equilibrated and the baseline is stable, click Run
Method in the Sample Info page to start the injection. Alternatively, click OK,
and when ready, click the Start Single Sample button above the system
diagram.
6 The injection is made, and the chromatogram appears in the Online Plot. The
data acquisition will stop when the Stop Time is reached.
The chromatogram should look similar to Figure 33 on page 101 — the time
axis will be longer, however, as this was run under four times faster
conditions in the next section.
NOTE
If changes have been made to a loaded method and not yet saved, this is indicated by a
yellow asterisk on the method status icon. The injection can be made without first saving
those parameters changes. The ChemStation always stores a copy of the acquisition
parameters in the data file as ACQ.TXT to ensure the preservation of the original method
parameters.